Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells

Autor: Etsu Tashiro, Masaya Imoto, Shigeyuki Magi, Masato Kasamatsu, Yuya Saeki
Rok vydání: 2013
Předmět:
MAPK/ERK pathway
Cell biology
Cell signaling
MAP Kinase Signaling System
Physiology
Cell
lcsh:Medicine
Cell Migration
Biology
Signal transduction
ERK signaling cascade
Biochemistry
Glycogen Synthase Kinase 3
Epidermal growth factor
Cell Movement
Cell Line
Tumor
Growth Factors
Chemical Biology
Molecular Cell Biology
Basic Cancer Research
medicine
Medicine and Health Sciences
Humans
AKT signaling cascade
lcsh:Science
Extracellular Signal-Regulated MAP Kinases
PI3K/AKT/mTOR pathway
Multidisciplinary
Epidermal Growth Factor
Biology and life sciences
Endocrine Physiology
Systems Biology
lcsh:R
JNK Mitogen-Activated Protein Kinases
Proteins
Signaling cascades
Cell migration
Cell Motility
medicine.anatomical_structure
Oncology
Cell culture
Small Molecules
Cancer cell
lcsh:Q
Cellular Types
Research Article
Biotechnology
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 5, p e96776 (2014)
ISSN: 1932-6203
Popis: To explore the diversity and consistency of the signaling pathways that regulate tumor cell migration, we chose three human tumor cell lines that migrated after treatment with EGF. We then quantified the effect of fifteen inhibitors on the levels of expression or the phosphorylation levels of nine proteins that were induced by EGF stimulation in each of these cell lines. Based on the data obtained in this study and chemical-biological assumptions, we deduced cell migration pathways in each tumor cell line, and then compared them. As a result, we found that both the MEK/ERK and JNK/c-Jun pathways were activated in all three migrating cell lines. Moreover, GSK-3 and p38 were found to regulate PI3K/Akt pathway in only EC109 cells, and JNK was found to crosstalk with p38 and Fos related pathway in only TT cells. Taken together, our analytical system could easily distinguish between the common and cell type-specific pathways responsible for tumor cell migration.
Databáze: OpenAIRE
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