Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19 + rP39) against B. abortus 544 and B. melitensis 16M infection in murine model

Autor: Amit Kumar Singh, Harsh Vardhan Batra, K. Balakrishna, Ganesh Tadepalli, H.S. Murali
Rok vydání: 2016
Předmět:
Zdroj: Molecular Immunology. 71:34-41
ISSN: 0161-5890
DOI: 10.1016/j.molimm.2016.01.001
Popis: In this study, the immunogenicity and protective efficacy of recombinant proteins Omp19 (rO) and P39 (rP) from Brucella abortus were evaluated individually and compared with the cocktail protein (rO+rP) against B. abortus 544 and Brucella melitensis 16M infection in BALB/c mouse model. Intra-peritoneal (I.P.) immunization with rO+rP cocktail developed substantially higher antibody titers predominant with Th1 mediated isotypes (IgG2a/2b). Western blot analysis using anti-rO+rP antibodies showed specific reactivity with native Omp19 (19 kDa) and P39 (39 kDa) among whole cell proteins of B. abortus and B. melitensis. Splenocytes extracted from rO+rP immunized mice induced significantly (P
Databáze: OpenAIRE