Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19 + rP39) against B. abortus 544 and B. melitensis 16M infection in murine model
Autor: | Amit Kumar Singh, Harsh Vardhan Batra, K. Balakrishna, Ganesh Tadepalli, H.S. Murali |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Lipoproteins Blotting Western Immunology Brucella Vaccine Brucella abortus Spleen Brucellosis Microbiology Mice 03 medical and health sciences Brucella melitensis medicine Splenocyte Animals Molecular Biology Antigens Bacterial Mice Inbred BALB C Vaccines Synthetic biology Immunogenicity Antibody titer biology.organism_classification Antibodies Bacterial Recombinant Proteins Disease Models Animal Titer 030104 developmental biology medicine.anatomical_structure Periplasmic Binding Proteins biology.protein Female Antibody Bacterial Outer Membrane Proteins |
Zdroj: | Molecular Immunology. 71:34-41 |
ISSN: | 0161-5890 |
DOI: | 10.1016/j.molimm.2016.01.001 |
Popis: | In this study, the immunogenicity and protective efficacy of recombinant proteins Omp19 (rO) and P39 (rP) from Brucella abortus were evaluated individually and compared with the cocktail protein (rO+rP) against B. abortus 544 and Brucella melitensis 16M infection in BALB/c mouse model. Intra-peritoneal (I.P.) immunization with rO+rP cocktail developed substantially higher antibody titers predominant with Th1 mediated isotypes (IgG2a/2b). Western blot analysis using anti-rO+rP antibodies showed specific reactivity with native Omp19 (19 kDa) and P39 (39 kDa) among whole cell proteins of B. abortus and B. melitensis. Splenocytes extracted from rO+rP immunized mice induced significantly (P |
Databáze: | OpenAIRE |
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