Presence of cardiac alpha-myosin correlates with histochemical myosin Ca2+ ATPase activity in rabbit masseter muscle
| Autor: | Antoon F.M. Moorman, W. A. Weijs, J. J. Bredman |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Alpha-Myosin
ATPase Calcium-Transporting ATPases Cross Reactions Myosins Masseter muscle Fetus Pregnancy Myosin Atpase activity Animals chemistry.chemical_classification Lagomorpha biology Chemistry Masseter Muscle Myocardium Rabbit (nuclear engineering) Cell Biology Hydrogen-Ion Concentration biology.organism_classification Immunohistochemistry Enzyme Biochemistry biology.protein Female Rabbits Anatomy |
| Zdroj: | Histochemical journal, 24(5), 260-265. Kluwer Academic Publishers |
| ISSN: | 0018-2214 |
| DOI: | 10.1007/bf01046840 |
| Popis: | A combined enzyme-histochemical (ATPase reactivity) and immunohistochemical study has been performed on sections of rabbit masseter muscle. The majority of the fibres previously designated as type IIC and/or type I according to their ATPase activity were found to contain 'cardiac' alpha-myosin heavy chain in addition to other myosin heavy chains. All alpha-myosin heavy chain-containing fibres reveal ATPase activity after pre-incubation at pH 4.2-4.6 similar to that of the classical type I fibres, while, in that pH range, limb type IIC fibres show intermediate ATPase activity. One group of these fibres reveal ATPase activity after pre-incubation at pH 10.1-10.3 as well, but not at pH 10.4-10.5. These fibres contain exclusively either alpha- or alpha- and I-myosin heavy chains but do not contain the IIA-myosin heavy chain. The second part of the fibres reveals ATPase activity after treatment within the whole alkaline pre-incubation range (pH 10.1-10.5) and these fibres contain alpha-myosin and IIA-myosin but no I-myosin heavy chain. It is concluded that the classical IIC fibre type is not present in the rabbit masseter muscle. Furthermore, ATPase reactivity does not allow us to distinguish fibres on their myosin heavy chain content in rabbit masseter muscle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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