| Autor: |
Mehdi Dadmehr, Sahar Cheraghi Shahi, Mitra Malekkiani, Behnaz Korouzhdehi, Abolfazl Tavassoli |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Food Chemistry. 402:134212 |
| ISSN: |
0308-8146 |
| DOI: |
10.1016/j.foodchem.2022.134212 |
| Popis: |
Aflatoxin as the potent carcinogenic mycotoxin had been received great attention for detection in food industry and safety. Due to low quantity of aflatoxin in food samples, there is a need to develop a sensitive method toward its detection. In the present study, an aflatoxin B1 (AFB1) specific aptasensor with internal complementary sequence was developed for detection of AFB1. The FAM-functionalized aptamer was deposited on the surface of graphene oxide decorated with gold nanoparticles (GO/AuNPs) and following formation of heteroduplex stem-loop structure led to fluorescence quenching of FAM-labeled aptamer. After incubation of proposed aptasensor with AFB1, the aptamer-AFB1 complex resulted to denaturation in stem-loop structure of aptamer which caused restoration of single strand aptamer and recovery of fluorescence. The assay showed a convenient detection response in the range of 0.5-20 pg/mL with a very low detection limit (0.1 pg/mL) and it was highly selective for AFB1 over other mycotoxins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect