MUC1 in human and murine mammary carcinoma cells decreases the expression of core 2 1,6-N-acetylglucosaminyltransferase and -galactoside 2,3-sialyltransferase
Autor: | Véronique Piller, Tomasz Owczarek, Piotr Dziegiel, Maciej Ugorski, Alicja Solatycka, Friedrich Piller, Marzena Podhorska-Okolow, Lukasz Wojciech, Bartosz Pula |
---|---|
Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Department of Histology and Embryology, Wroclaw Medical University |
Rok vydání: | 2012 |
Předmět: |
Glycosylation
Galectin 3 Biochemistry Immunoenzyme Techniques Small hairpin RNA Mice 0302 clinical medicine RNA interference Tumor Cells Cultured RNA Small Interfering skin and connective tissue diseases MUC1 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Carcinoma Ductal Breast Flow Cytometry Galactosyltransferases 3. Good health 030220 oncology & carcinogenesis Female E-Selectin beta-Galactoside alpha-2 3-Sialyltransferase Sialyltransferase Blotting Western Breast Neoplasms N-Acetylglucosaminyltransferases Real-Time Polymerase Chain Reaction digestive system 03 medical and health sciences Antigen Complementary DNA Cell Adhesion Animals Humans Antigens Tumor-Associated Carbohydrate [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger neoplasms 030304 developmental biology Messenger RNA Mucin-1 Molecular biology N-Acetylneuraminic Acid Sialyltransferases biological factors digestive system diseases biology.protein Neuraminidase |
Zdroj: | Glycobiology Glycobiology, Oxford University Press (OUP), 2012, 22 (8), pp.1042-54. ⟨10.1093/glycob/cws075⟩ |
ISSN: | 1460-2423 0959-6658 |
Popis: | International audience; A good correlation between the expression of mucin1 (MUC1) and T antigen was found in breast cancer tumors and breast cancer cell lines, especially after treatment with neuraminidase. The association between the appearance of T antigen and the overexpression of MUC1 was further confirmed by transfecting MDA-MB-231 cells and murine 4T1 mammary carcinoma cells with cDNA for MUC1 and using an RNAi approach to inhibit the expression of MUC1 gene in T47D cells. Furthermore, we discovered that in 4T1 cells which express the sialyl Le(X) antigen, overexpression of MUC1 caused not only appearance of T antigen, but also loss of the sialyl Le(X) structure. As the observed changes in O-glycan synthesis can be associated with changes in the expression of specific glycosyltransferases, core 1 β1,3-galactosyltransferase, core 2 β1,6-N-acetylglucosaminyltransferase (C2GnT1) and β-galactoside α2,3-sialyltransferase (ST3Gal I), we studied their expression in parental, vector-transfected and MUC1-transfected MDA-MB-231 and 4T1 cells as well as T47D cells transduced with small hairpin RNA targeted MUC1 mRNA. It was found that the expression of C2GnT1 and ST3Gal I is highly decreased in MUC1-expressing MDA-MB-231 and 4T1 cells and increased in T47D cells with suppressed expression of MUC1. Therefore, we found that changes in the structure of O-linked oligosaccharides, resulting in the occurrence of T antigen, are at least partially associated with MUC1 overexpression which down-regulates the expression of C2GnT1 and ST3Gal I. We showed also that the overexpression of MUC1 in 4T1 cells changes their adhesive properties, as MUC1-expressing cells do not adhere to E-selectin, but bind galectin-3. |
Databáze: | OpenAIRE |
Externí odkaz: |