Transcription Profile Analyses Identify Genes and Pathways Central to Root Cap Functions in Maize

Autor: George Tsai, Charles Chilcott, Lewis J. Feldman, Kyungpil Kim, Stanley Lee, Tong Zhu, Keni Jiang, Haiyan Huang, Shibo Zhang
Rok vydání: 2006
Předmět:
DNA
Complementary
Time Factors
Transcription
Genetic
Arabidopsis
Carbohydrates
Down-Regulation
Cyclopentanes
Plant Science
Models
Biological
Plant Roots
Zea mays
Gene Expression Regulation
Plant
Transcription (biology)
Gene expression
Genetics
Oxylipins
RNA
Messenger
Gene
Root cap
Plant Physiological Phenomena
Oligonucleotide Array Sequence Analysis
Laser capture microdissection
Regulation of gene expression
Internet
biology
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Lasers
Cell Membrane
Nucleic Acid Hybridization
food and beverages
Biological Transport
Oryza
General Medicine
Ethylenes
Meristem
biology.organism_classification
Molecular biology
Hormones
Up-Regulation
Gene expression profiling
Gene Expression Regulation
Carbohydrate Metabolism
RNA
Microdissection
Agronomy and Crop Science
Signal Transduction
Zdroj: Plant Molecular Biology. 60:343-363
ISSN: 1573-5028
0167-4412
DOI: 10.1007/s11103-005-4209-4
Popis: Affymetrix GeneChips arrayed with about one-half (~23K) of the rice genes were used to profile gene transcription activity in three tissues comprising the maize root tip; the proximal meristem (PM), the quiescent center (QC), and the root cap (RC). Here we analyze the gene transcription profile of the RC, compared to both the PM and the QC, from three biological replicates. In the RC, a total of 669 genes were identified as being differentially upregulated, and 365 differentially downregulated. Real-time quantitative RT-PCR analysis was used to confirm upregulated genes in the RC. In addition, using the technique of laser microdissection (LMD) we localized upregulated gene expression to the lateral RC cells. Taken as a whole, transcription profile analyses revealed the upregulation in the maize RC of clusters of genes linked to major metabolic processes and pathways, including: (1) transport, both the export of carbohydrates and the uptake of nutrients; (2) sensing and responding to (often stressful) biotic and abiotic environmental stimuli; (3) integrating the responses of at least 3 major growth regulators (auxin, ethylene, jasmonic acid); (4) processing the large amount of carbohydrate transported into the RC. Although the profile data are derived using heterologous rice GeneChips, with about half of the total rice gene set, this study, nevertheless, provides a genomic scale characterization of the entire RC, and serves as a new platform from which to advance studies of the network of pathways operating in the maize RC.
Databáze: OpenAIRE
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