TP53 mutations in peripheral mature T and NK cell lymphomas: a whole-exome sequencing study with correlation to p53 expression
| Autor: | Chun-Kai Liao, Ming-Chung Wang, Hui-Shan Huang, Huey-Ling You, Ting-Ting Liu, Wan-Ting Huang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Angioimmunoblastic T-cell lymphoma Cell Peripheral T-cell lymphoma not otherwise specified Biology medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences Exome Sequencing medicine Humans Exome sequencing In Situ Hybridization Aged Aged 80 and over Cancer Lymphoma T-Cell Peripheral Middle Aged medicine.disease Molecular biology Lymphoma Killer Cells Natural Lymphoma Extranodal NK-T-Cell 030104 developmental biology medicine.anatomical_structure Mutation Immunohistochemistry Female Tumor Suppressor Protein p53 Carcinogenesis |
| Zdroj: | Human pathology. 80 |
| ISSN: | 1532-8392 |
| Popis: | Peripheral mature T and NK cell lymphomas consist of a heterogeneous group of neoplasms with cytogenetic and molecular diversities. TP53 mutation is involved in the events of tumorigenesis and present in a variety of cancer subtypes. However, TP53 mutation in peripheral mature T and NK cell lymphomas has not been extensively investigated. In this study, 57 formalin-fixed paraffin-embedded samples were collected for whole-exome sequencing and immunohistochemical study. Ten cases had TP53 mutation variant allele frequency (VAF) of more than 5%, predominantly in intestinal T-cell lymphoma (60%), peripheral T-cell lymphoma, not otherwise specified (22.2%), and extranodal nasal-type NK/T-cell lymphoma (21.7%). Six of 16 (37.5%) cases with 72Pro homozygous genotype showed higher frequency of TP53 mutation VAF > 5% than did those with 72Arg homozygous genotype (P = .044). To a variable extent, overexpression of p53 was observed in 11 of 56 (19.6%) tumors. The percentage of tumor cells with strong p53 staining was positively correlated with TP53 mutation VAF (R2 = 0.95, P < .001). TP53 mutation and p53 expression tended to indicate poor prognosis (P = .054 and P = .009, respectively). In conclusion, we demonstrated TP53 mutation and p53 expression in subtypes of peripheral mature T and NK cell lymphomas and found positive correlation of this immunostaing pattern with the status of TP53 mutation. |
| Databáze: | OpenAIRE |
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