AuNPs-functionalized PANABA-MWCNTs nanocomposite-based impedimetric immunosensor for 2,4-dichlorophenoxy acetic acid detection
| Autor: | Francesca Gallo, Gabriele Favero, Andrea D'Annibale, Riccarda Antiochia, Paolo Bollella, Giovanni Fusco, Federica Ietto, Franco Mazzei, Cristina Tortolini, Antonella De Mico |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Polymers
Biomedical Engineering Biophysics Analytical chemistry Metal Nanoparticles Biosensing Techniques 02 engineering and technology 01 natural sciences Nanomaterials chemistry.chemical_compound Acetic acid Aniline Limit of Detection Polyaniline 2 4-Dichlorophenoxy acetic acid au nanoparticles Impedimetric immunosensor nanocomposite polyaniline screen printed electrodes biotechnology biophysics biomedical engineering electrochemistry Electrochemistry Electrodes Conductive polymer Aniline Compounds Nanocomposite Nanotubes Carbon Chemistry 010401 analytical chemistry Electrochemical Techniques General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Dielectric spectroscopy Colloidal gold Dielectric Spectroscopy Gold 2 4-Dichlorophenoxyacetic Acid 0210 nano-technology Biotechnology Nuclear chemistry |
| Popis: | In this work, we developed an impedimetric label-free immunosensor for the detection of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) herbicide either in standard solution and spiked real samples. For this purpose, we prepared by electropolymerization a conductive polymer poly-(aniline-co-3-aminobenzoic acid) (PANABA) then we immobilized anti-2,4-D antibody onto a nanocomposite AuNPs-PANABA-MWCNTs employing the carboxylic moieties as anchor sites. The nanocomposite was synthesized by electrochemical polymerization of aniline and 3-aminobenzoic acid, in the presence of a dispersion of gold nanoparticles, onto a multi-walled carbon nanotubes-based screen printed electrode. Aniline-based copolymer, modified with the nanomaterials, allowed to enhance the electrode conductivity thus obtaining a more sensitive antigen detection. The impedimetric measurements were carried out by electrochemical impedance spectroscopy (EIS) in faradic condition by using Fe(CN)63-/4- as redox probe. The developed impedimetric immunosensor displayed a wide linearity range towards 2,4-D (1-100ppb), good repeatability (RSD 6%), stability and a LOD (0.3ppb) lower than herbicide emission limits. |
| Databáze: | OpenAIRE |
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