Eldecalcitol (ED-71), an analog of 1α,25-dihydroxyvitamin D3 as a potential anti-cancer agent for oral squamous cell carcinomas
Autor: | Emiko Usui, Shigeaki Toratani, Tetsuji Okamoto, Tomoaki Shintani, S.N.Z. Rosli, Yasutaka Hayashido, Y.F. Choon, F Takatsu |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Calcitriol Angiogenesis Endocrinology Diabetes and Metabolism Clinical Biochemistry Cell Gingiva Mice Nude Antineoplastic Agents Biochemistry Culture Media Serum-Free 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Cell Line Tumor medicine Animals Humans Vitamin D Vitamin D3 24-Hydroxylase Molecular Biology Mice Inbred BALB C Dose-Response Relationship Drug business.industry Cancer Cell Biology Fibroblasts medicine.disease 030104 developmental biology medicine.anatomical_structure Epidermoid carcinoma Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Carcinoma Squamous Cell Molecular Medicine Mouth Neoplasms business A431 cells Neoplasm Transplantation medicine.drug |
Zdroj: | The Journal of steroid biochemistry and molecular biology. 164 |
ISSN: | 1879-1220 |
Popis: | We have previously reported that 1,25(OH)2D3 inhibits NF-κB activity and thus inhibits growth of OSCC cells in serum-free culture and down-regulates HBp17/FGFBP-1 expression, which is important for cancer cell growth and angiogenesis. Here, we have investigated the effects of ED-71, an analog of vitamin D3 (VD) on OSCC cell lines in serum-free culture. It is known that ED-71 has a stronger inhibitory effect on bone resorption compared to VD and other VD analogs. To the best of our knowledge, there was no report examining the potential of ED-71 as an anti-cancer agent for OSCC. We found that ED-71 is able to inhibit the growth of cancer cell lines at a concentration of hundred times lower than calcitriol. As Cyp24A1 was reportedly induced in cancer cells, we measured the expression of CYP24A1 in OSCC cell lines (NA and UE), A431 epidermoid carcinoma and normal fibroblast cell (gfi) in serum-free culture. As a result, CYP24A1 mRNA and the protein expression in the OSCC cells treated with ED-71 increased in a dose-dependent manner. However, in vivo experiment, in which the A431 cells were implanted in mice, tumor formation was reduced by the ED-71 treatment with no significant difference between Cyp24A1 expression in the tumors of ED-71-treated and control group, as analyzed by western blotting and immunohistochemistry. These results suggest that ED-71 is a potential anti-cancer agent for OSCC. |
Databáze: | OpenAIRE |
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