Distinct contribution of hyperbaric oxygen therapy to human neutrophil function and antibiotic efficacy against Staphylococcus aureus

Autor:	Christian Johann Lerche, Lars Christophersen, Anne Sofie Laulund, Anders Woetmann, Franziska A. Schwartz, Claus Moser, Niels Høiby, Peter Østrup Jensen
Rok vydání:	2021
Předmět:	Microbiology (medical) Staphylococcus aureus medicine.drug_class Neutrophils Antibiotics Microbial Sensitivity Tests Penicillins Pharmacology Hyperoxia In Vitro Techniques medicine.disease_cause Benzylpenicillin Pathology and Forensic Medicine Ciprofloxacin medicine Tobramycin Immunology and Allergy Humans chemistry.chemical_classification Reactive oxygen species Hyperbaric Oxygenation business.industry General Medicine Staphylococcal Infections Combined Modality Therapy Anti-Bacterial Agents Penicillin chemistry business Reactive Oxygen Species Oxidative stress medicine.drug
Zdroj:	APMIS : acta pathologica, microbiologica, et immunologica ScandinavicaReferences. 129(9)
ISSN:	1600-0463
Popis:	Staphylococcus aureus (SA) causes superficial and severe endovascular infections. The present in vitro study investigates the anti-SA mechanisms of hyperbaric oxygen therapy (HBOT) on direct bacterial killing, antibiotic potentiation, and polymorphonuclear leukocyte (PMN) enhancement. SA was exposed to isolated human PMNs, tobramycin, ciprofloxacin, or benzylpenicillin. HBOT was used as one 90-min session. Bacterial survival was evaluated after 4 h by quantitative bacteriology. PMN functionality as reactive oxygen species (ROS) production was measured by means of dihydrorhodamine 123 analysis. We showed that HBOT exhibits significant direct anti-SA effects. HBOT increased the anti-SA effects of PMNs by 18% after PMA stimulation (p = 0.0004) and by 15% in response to SA (p = 0.36). HBOT showed an additive effect as growth reductions of 26% to sub-MICs of tobramycin (p = 0.0057), 44% to sub-MICs of ciprofloxacin (p = 0.0001), and 26% to sub-MICs of penicillin (p = 0.038). The present in vitro study provides evidence that HBOT has differential mechanisms mediating its anti-SA effects. Our observation supports the clinical possibility for adjunctive HBOT to augment the host immune response and optimize the efficacy of antibiotic treatments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1b8b523b3aa000746b1de3d34942703 https://pubmed.ncbi.nlm.nih.gov/34120378 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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