Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia
| Autor: | Hamid Reza Mollaei, Masood Ziaee, Mohammad Hasan Namaei, Davod Javanmard, Ahmad Tavakoli, Helya Sadat Mortazavi, Mohsen Moghoofei, Hadi Ghaffari, Seyed Hamidreza Monavari |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty PARV4 Parenteral transmission Parvovirus B19 Human parvovirus Iran 030204 cardiovascular system & hematology Virus law.invention 03 medical and health sciences 0302 clinical medicine law Internal medicine Epidemiology Prevalence Medicine Clinical significance Hemophilia Polymerase chain reaction Whole blood biology business.industry Parvovirus Hematology biology.organism_classification 030104 developmental biology Original Article business |
| Zdroj: | Blood research |
| ISSN: | 2288-0011 2287-979X |
| Popis: | Background Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resistance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovirus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran. Methods This was a cross-sectional epidemiological study comprising nearly all people with hemophilia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time polymerase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR. Results In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients (P |
| Databáze: | OpenAIRE |
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