Use of antioxidant nanoparticles to reduce oxidative stress in blood storage
| Autor: | Amir Amani, Mehdi Barati, Saeid Barzegar, Maryam Mohammadi Najafabadi, Javad Hashemi, Ali Akbar Pourfathollah, Mahsa Rahgoshay, Amir Asri Kojabad, Rima Manafi Shabestari, Majid Safa, Mohammad Reza Rezvany, Farhad Zaker, Alireza Abbaspour |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.medical_treatment Biomedical Engineering Bioengineering Phosphatidylserines Hematocrit medicine.disease_cause Applied Microbiology and Biotechnology Antioxidants Andrology chemistry.chemical_compound Drug Discovery medicine chemistry.chemical_classification Glutathione Peroxidase medicine.diagnostic_test Superoxide Dismutase Chemistry Process Chemistry and Technology Glutathione peroxidase General Medicine Glutathione Catalase medicine.disease Malondialdehyde Hemolysis Oxidative Stress Blood Preservation Nanoparticles Molecular Medicine Hemoglobin Oxidative stress Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 69:1712-1722 |
| ISSN: | 1470-8744 0885-4513 |
| DOI: | 10.1002/bab.2240 |
| Popis: | Oxidative damage by free radicals has a negative effect on blood quality during storage. Antioxidant nanoparticles can prevent oxidative stress. We use SOD-CAT-Alb-PEG-PLGA- nanoparticles to reduce the effects of oxidative stress in blood storage. Electrospray was employed to prepare nanoparticles. Nanoparticles entered the test bags and were kept for 35 days from the time of donation under standard conditions. On target days, experiments were performed on the samples taken. The examination included blood smear, red blood cells count, hemoglobin, hematocrit, K, Fe, glutathione peroxidase, glutathion reductase, glucose-6-phosphate dehydrogenase, prooxidant-antioxidant balance, malondialdehyde, and flow cytometric assay for phosphatidylserine. The repeated measures analysis was performed on samples every week. Morphological changes were less in the test group compared to the control. The quantitative hemolysis profile test showed significant changes in the test and control groups (p < 0.05) in consecutive weeks except for K and Fe. Oxidative stress parameters too showed a significant change during the target days of the examination (p < 0.05). Also, the phosphatidylserine expression was increased in control groups more than test in consecutive weeks (p < 0.05). It seems that the use of antioxidant nanoparticles improves the quality of stored red blood cells and can prevent posttransfusion complications and blood loss by reducing oxidative stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text