Prevotella intermedia lipopolysaccharide stimulates release of tumor necrosis factor-alpha through mitogen-activated protein kinase signaling pathways in monocyte-derived macrophages
| Autor: | Ju-Youn Lee, Su-Hwa Shin, In-Soon Choi, Sung-Jo Kim, Jeom-Il Choi, Eun Gyung Kim, Eun-Young Choi |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
MAPK/ERK pathway Lipopolysaccharides medicine.medical_specialty MAP Kinase Signaling System medicine.medical_treatment Immunology Blotting Western Gene Expression Biology Microbiology Prevotella intermedia Cell Line Internal medicine medicine Immunology and Allergy Humans RNA Messenger Protein kinase A Kinase Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Monocyte Macrophages General Medicine biology.organism_classification Molecular biology Infectious Diseases Cytokine medicine.anatomical_structure Endocrinology Tumor necrosis factor alpha Signal transduction |
| Zdroj: | FEMS immunology and medical microbiology. 51(2) |
| ISSN: | 0928-8244 |
| Popis: | The purpose of this study was to investigate the effects of lipopolysaccharide from Prevotella intermedia, a major cause of inflammatory periodontal disease, on the production of tumor necrosis factor (TNF)-alpha and the expression of TNF-alpha mRNA in differentiated THP-1 cells, a human monocytic cell line. The potential involvement of the three main mitogen-activated protein kinase (MAPK) signaling pathways in the induction of TNF-alpha production was also investigated. Lipopolysaccharide from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. It was found that P. intermedia lipopolysaccharide can induce TNF-alpha mRNA expression and stimulate the release of TNF-alpha in differentiated THP-1 cells without additional stimuli. Treatment of the cells with P. intermedia lipopolysaccharide resulted in a simultaneous activation of three MAPKs [extracellular signal-related kinase 1/2 (ERK1/2), c-Jun N-terminal kinase 1/2 (JNK1/2) and p38]. Pretreatment of the cells with MAPK inhibitors effectively suppressed P. intermedia lipopolysaccharide-induced TNF-alpha production without affecting the expression of TNF-alpha mRNA. These data thus provided good evidence that the MAPK signaling pathways are required for the regulation of P. intermedia lipopolysaccharide-induced TNF-alpha synthesis at the level of translation more than at the transcriptional level. |
| Databáze: | OpenAIRE |
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