Recruitment of a Ribosomal Release Factor for Light- and Stress-Dependent Regulation of petB Transcript Stability in Arabidopsis Chloroplasts
| Autor: | Lina Lezhneva, Rhea Stoppel, Jörg Meurer, Serena Schwenkert, Susanne Felder, Peter Westhoff, Karin Meierhoff, Salar Torabi |
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| Rok vydání: | 2011 |
| Předmět: |
Ribosomal Proteins
Chloroplasts Light RNA Stability Molecular Sequence Data Arabidopsis Plant Science Electron Transport Gene Expression Regulation Plant Stress Physiological Protein Isoforms Amino Acid Sequence Plastid Research Articles Phylogeny Plant Proteins Genetics biology Arabidopsis Proteins fungi Intron RNA-Binding Proteins food and beverages Cell Biology Ribosomal RNA biology.organism_classification Stop codon Chloroplast Chloroplast stroma Cytochrome b6f Complex Multigene Family Mutation Codon Terminator Release factor Sequence Alignment |
| Zdroj: | The Plant Cell. 23:2680-2695 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.111.085324 |
| Popis: | Land plant genomes encode four functional ribosomal peptide chain release factors (Prf) of eubacterial origin, two (PrfA and PrfB homologs) for each endosymbiotic organelle. Formerly, we have shown that the Arabidopsis thaliana chloroplast-localized PrfB homolog, PrfB1, is required not only for termination of translation but also for stabilization of UGA stop codon-containing chloroplast transcripts. A previously undiscovered PrfB-like protein, PrfB3, is localized to the chloroplast stroma in a petB RNA-containing complex and found only in vascular plants. Highly conserved positions of introns unequivocally indicate that PrfB3 arose from a duplication of PrfB1. Notably, PrfB3 is lacking the two most important tripeptide motifs characteristic for all eubacterial and organellar PrfB homologs described so far: the stop codon recognition motif SPF and the catalytic center GGQ for peptidyl-tRNA hydrolysis. Complementation studies, as well as functional and molecular analyses of two allelic mutations in Arabidopsis, both of which lead to a specific deficiency of the cytochrome b₆f complex, revealed that PrfB3 is essentially required for photoautotrophic growth. Plastid transcript, polysome, and translation analyses indicate that PrfB3 has been recruited in vascular plants for light- and stress-dependent regulation of stability of 3' processed petB transcripts to adjust cytochrome b₆ levels. |
| Databáze: | OpenAIRE |
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