Long non-coding RNAs expression profile and functional analysis of acute ischemic stroke
| Autor: | Jiajun Chen, Tianji Shi, Ben Yang, Jingqi Feng, Miao Hao, Yingyu Zhang, Jia Li |
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| Rok vydání: | 2020 |
| Předmět: |
Male
China acute ischemia stroke Observational Study Down-Regulation Disease Bioinformatics Real-Time Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Adipokines Medicine Humans 030212 general & internal medicine RNA Messenger Stroke Gene Adipocytokine Signaling Pathway Aged Ischemic Stroke long non-coding RNA business.industry Case-control study RNA High-Throughput Nucleotide Sequencing General Medicine Middle Aged medicine.disease Magnetic Resonance Imaging pathway analysis Up-Regulation Gene Ontology 030220 oncology & carcinogenesis Case-Control Studies Female RNA Long Noncoding business Tomography X-Ray Computed Function (biology) Biomarkers Research Article |
| Zdroj: | Medicine |
| ISSN: | 1536-5964 |
| Popis: | Long non-coding RNAs (lncRNAs) have been evidenced to be associated with the development of multiple diseases. However, the expression pattern and function of lncRNAs in acute ischemic stroke remain unclear. To determine the differential expression of lncRNAs in acute ischemic stroke, we analyzed the expression profile of lncRNAs by high-throughput sequencing analysis. Gene Ontology (GO) and pathway analyses were employed to analyze the gene function and identify enriched pathways of the differentially expressed lncRNAs. We also built an lncRNA-mRNA expression correlation network and verified the interactions of selected lncRNAs in acute ischemic stroke. To further confirm the results of the expression profile, 6 differentially expressed lncRNAs were randomly selected and quantitative RT-PCR (qRT-PCR) performed. We identified 44,578 aberrantly expressed lncRNAs, including 228 upregulated and 16 downregulated lncRNAs. The qRT-PCR results showed that ENSG00000269900, ENSG00000196559, ENSG00000202198, ENSG00000226482, ENSG00000260539 (up), and XLOC_013994_2 (down) were abnormally expressed, which was consistent with the sequencing results. The upregulated expression of lncRNA ENSG00000226482 may activate the adipocytokine signaling pathway, resulting in acute ischemia stroke. In summary, we analyzed the lncRNAs expression profile in acute ischemic stroke patients and identified the functions and enriched metabolic pathways, proposing new insights into the diagnostic and therapeutic biomarkers for this disease. |
| Databáze: | OpenAIRE |
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