Antifungal Activity of Naphthoquinoidal Compounds In Vitro against Fluconazole-Resistant Strains of Different Candida Species: A Special Emphasis on Mechanisms of Action on Candida tropicalis

Autor: Hemerson Iury Ferreira Magalhães, Emilay B. T. Diogo, Tatiane S. C. Carvalho, Marina Duarte Pinto Lobo, Rosana de Sousa Campos, Hélio Vitoriano Nobre Júnior, João Batista de Andrade Neto, Thalles B. Grangeiro, Bruno C. Cavalcanti, Rose Anny Costa Silva, Felipe A. R. Rodrigues, Danielle Macedo Gaspar, Manoel Odorico de Moraes, Janaína T. Siebra, Cecília Rocha da Silva, Maria A. S. Neta, Eufrânio N. da Silva Júnior
Rok vydání: 2014
Předmět:
Antifungal Agents
lcsh:Medicine
Plant Science
Biochemistry
Candida tropicalis
Drug Discovery
Medicine and Health Sciences
DNA
Fungal
Fungal Biochemistry
lcsh:Science
Fluconazole
Candida
Membrane Potential
Mitochondrial
chemistry.chemical_classification
Multidisciplinary
Molecular Structure
biology
Candidiasis
Obstetrics and Gynecology
RNA
Ribosomal
5.8S
Infectious Diseases
Toxicity
Intracellular
Research Article
Biotechnology
medicine.drug
Drug Research and Development
Cell Survival
DNA damage
Urology
Molecular Sequence Data
Sexually Transmitted Diseases
Microbial Sensitivity Tests
Phosphatidylserines
Mycology
Microbiology
Cell Line
Drug Resistance
Fungal
DNA
Ribosomal Spacer
medicine
Animals
Pharmacology
Reactive oxygen species
Genitourinary Infections
lcsh:R
Organisms
Fungi
Biology and Life Sciences
Sequence Analysis
DNA
Fibroblasts
biology.organism_classification
Yeast
In vitro
Models
Chemical
chemistry
Fluconazol
Women's Health
lcsh:Q
Clinical Medicine
Reactive Oxygen Species
DNA Damage
Naphthoquinones
Zdroj: PLoS ONE, Vol 9, Iss 5, p e93698 (2014)
Repositório Institucional da Universidade Federal do Ceará (UFC)
Universidade Federal do Ceará (UFC)
instacron:UFC
PLoS ONE
ISSN: 1932-6203
Popis: In recent decades, the incidence of candidemia in tertiary hospitals worldwide has substantially increased. These infections are a major cause of morbidity and mortality; in addition, they prolong hospital stays and raise the costs associated with treatment. Studies have reported a significant increase in infections by non-albicans Candida species, especially C. tropicalis. The number of antifungal drugs on the market is small in comparison to the number of antibacterial agents available. The limited number of treatment options, coupled with the increasing frequency of cross-resistance, makes it necessary to develop new therapeutic strategies. The objective of this study was to evaluate and compare the antifungal activities of three semisynthetic naphthofuranquinone molecules against fluconazole-resistant Candida spp. strains. These results allowed to us to evaluate the antifungal effects of three naphthofuranquinones on fluconazole-resistant C. tropicalis. The toxicity of these compounds was manifested as increased intracellular ROS, which resulted in membrane damage and changes in cell size/granularity, mitochondrial membrane depolarization, and DNA damage (including oxidation and strand breakage). In conclusion, the tested naphthofuranquinones (compounds 1–3) exhibited in vitro cytotoxicity against fluconazole-resistant Candida spp. strains.
Databáze: OpenAIRE
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