Targeting of macromolecular carriers and liposomes by antibodies to myosin heavy chain
| Autor: | Vladimir P. Torchilin, Sean M. O'Donnell, Alexander L. Klibanov, Ban-An Khaw, Leaf Huang, Naseem Nossiff, Mikhail A. Slinkin |
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| Rok vydání: | 1991 |
| Předmět: |
Pulmonary and Respiratory Medicine
Macromolecular Substances Polymers medicine.drug_class Physiology Myocardial Infarction Polyethylene glycol Deferoxamine Myosins Monoclonal antibody Polyethylene Glycols Necrosis chemistry.chemical_compound Physiology (medical) Myosin medicine Animals Polylysine Chelation Chelating Agents Drug Carriers Liposome biology Myocardium Antibodies Monoclonal Cell Biology In vitro Radioimmunodetection chemistry Biochemistry Liposomes biology.protein Rabbits Antibody Cardiology and Cardiovascular Medicine Conjugate |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 261:60-65 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | Macromolecular carriers and liposomes were covalently coupled to monoclonal antibodies against cardiac myosin heavy chain. Deferoxamine-modified polymers bound tightly with 67Ga and68 Ga radioisotopes. Ternary deferoxamine-polylysine antibody conjugates specifically targeted the radioisotopes to a myosin-coated microplate. Scatchard analysis revealed a high affinity of the conjugate for the target with a Kas of ≈108 M-1. Liposomes that contained immobilized antimyosin antibodies were targeted specifically to the myosin-coated plate. Additional coating of these liposomes with polyethylene glycol reduced specific binding to the target in vitro. However, because of the presence of polyethylene glycol on the surface of liposomes, these liposomes had a long half-life and slowly cleared from the bloodstream after intravenous injection. These immunoliposomes showed up to 16- to 18-fold specific localization to the necrotic areas of the myocardium in rabbits with experimental infarction. antimyosin; chelating polymer; liposome; monoclonal antibody; myocardial infarction; radioimmunoimaging |
| Databáze: | OpenAIRE |
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