High-resolution imaging reveals microbial biofilms on patient urinary catheters despite antibiotic administration
| Autor: | Chloe L. Pinkner, Ana L. Flores-Mireles, Michael G. Caparon, Aaron J L Lynch, Jennifer N. Walker, Alana Desai, Scott J. Hultgren |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Bacteriuria Urinalysis medicine.drug_class Urology Urinary system medicine.medical_treatment Antibiotics 030232 urology & nephrology Fluorescent Antibody Technique Urinary Catheters Article Enterococcus faecalis Urinary catheterization 03 medical and health sciences 0302 clinical medicine Staphylococcus epidermidis Internal medicine Humans Medicine Bacteria biology medicine.diagnostic_test business.industry biology.organism_classification medicine.disease Anti-Bacterial Agents Catheter Biofilms 030220 oncology & carcinogenesis Microscopy Electron Scanning Equipment Contamination Female business |
| Zdroj: | World J Urol |
| ISSN: | 1433-8726 0724-4983 |
| DOI: | 10.1007/s00345-019-03027-8 |
| Popis: | PURPOSE: Catheter-associated urinary tract infections (CAUTIs) are a significant cause of morbidity worldwide, as they account for 40% of all hospital-associated infections. Microbial biofilm formation on urinary catheters (UCs) limits antibiotic efficacy, making CAUTI extremely difficult to treat. To gain insight into the spatiotemporal microbe interactions on the catheter surface we sought to determine how the presence or absence of bacteriuria prior to catheterization affects the organism that ultimately forms a biofilm on the UC and how long after catheterization they emerge. METHODS: Thirty UCs were collected from patients who received a urine culture prior to catheterization, a UC, and antibiotics as part of standard of care. Immunofluorescence imaging and scanning electron microscopy were used to visualize patient UCs. RESULTS: Most patients did not have bacteria in their urine (based on standard urinalysis) prior to catheterization, yet microbes were detected on the majority of UCs, even with dwell times of |
| Databáze: | OpenAIRE |
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