One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device
| Autor: | Jenny Aveyard, Robert Wilson, Maryam Mehrabi, Andrew R. Cossins, Helen Braven |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Pcr cloning
Oligonucleotides Metal Nanoparticles One-Step Polymerase Chain Reaction Sensitivity and Specificity Catalysis chemistry.chemical_compound Collodion Nucleic Acids Materials Chemistry Reagent Strips Chromatography Chemistry Oligonucleotide Metals and Alloys General Chemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Biochemistry Colloidal gold Covalent bond Ceramics and Composites Nucleic acid Gold Nitrocellulose |
| Zdroj: | Chemical communications (Cambridge, England). (41) |
| ISSN: | 1359-7345 |
| Popis: | Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip. |
| Databáze: | OpenAIRE |
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