Long Intergenic Nonprotein Coding RNA 173 Inhibits Tumor Growth and Promotes Apoptosis by Repressing Sphingosine Kinase 1 Protein Expression in Pancreatic Cancer
| Autor: | Yanan Li, Guangliang Gu, Xingxing Li, Bin Zhang, Jiageng Xiong, Yuqing Gu, Xiaojun Yang, Qian Li, Zhuyin Qian |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Apoptosis 03 medical and health sciences 0302 clinical medicine Western blot Cell Line Tumor Pancreatic cancer Biomarkers Tumor Genetics medicine Humans Neoplasm Invasiveness Molecular Biology Protein kinase B Cell Proliferation medicine.diagnostic_test biology Cell growth Kinase NF-kappa B RNA Cell Biology General Medicine medicine.disease Gene Expression Regulation Neoplastic Pancreatic Neoplasms Phosphotransferases (Alcohol Group Acceptor) 030104 developmental biology Sphingosine kinase 1 030220 oncology & carcinogenesis Cancer research biology.protein RNA Long Noncoding Proto-Oncogene Proteins c-akt |
| Zdroj: | DNA and Cell Biology. 40:757-775 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2020.6103 |
| Popis: | Pancreatic cancer is a common malignant tumor worldwide. Extensive studies have been conducted on the functional role of long noncoding RNAs in pancreatic cancer. In this study, long intergenic nonprotein coding RNA 173 (LINC00173) was highly expressed in pancreatic cancer tissues. In vitro functional experiments showed that LINC00173 overexpression inhibited the proliferation and invasion of pancreatic cancer cells and promoted cell apoptosis in MIA PaCa-2 and PANC-1 cells. RNA sequencing analysis and Western blot assays demonstrated that LINC00173 reduced the expression of sphingosine kinase 1 (SPHK1) and then inhibited the protein expression of activated phospho-protein kinase B (AKT) and NF-κB. In vivo functional assays also revealed that LINC00173 inhibited the growth of pancreatic cancer xenografts, repressed cell proliferation, promoted cell apoptosis, and inhibited SPHK1 expression. The combined results of this study indicate that LINC00173 inhibits pancreatic cancer progression by repressing SPHK1 expression. Improving LINC00173 may represent a therapeutic strategy for pancreatic cancer in the future. |
| Databáze: | OpenAIRE |
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