Expression of HLA-G in human cornea, an immune-privileged tissue
| Autor: | Philippe Moreau, Edgardo D. Carosella, Magali Le Discorde, Patrick Sabatier, Jean-Marc Legeais |
|---|---|
| Přispěvatelé: | Unité de Recherche en Hémato-Immunologie (SRHI - UMR E05), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Université Paris Diderot - Paris 7 (UPD7), Banque Française des Yeux (BFY), Association, Département d'ophtalmologie, Université Paris Descartes - Paris 5 (UPD5), Service de Recherche en Hémato-Immunologie (SRHI - UMR_E 05), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot - Paris 7 (UPD7)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_treatment Fluorescent Antibody Technique Corneal Diseases MESH: Histocompatibility Antigens Class I Cornea Corneal Transplantation 0302 clinical medicine HLA Antigens MESH: Reverse Transcriptase Polymerase Chain Reaction HLA-G Immunology and Allergy MESH: Corneal Diseases MESH: Fluorescent Antibody Technique MESH: HLA Antigens 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction General Medicine Immunohistochemistry 3. Good health medicine.anatomical_structure MESH: Corneal Transplantation [SDV.IMM]Life Sciences [q-bio]/Immunology Adult Keratoconus medicine.medical_specialty Pseudophakia [SDV.IMM] Life Sciences [q-bio]/Immunology medicine.drug_class Immunology MESH: Keratoconus HLA-C Antigens Human leukocyte antigen Biology Monoclonal antibody 03 medical and health sciences Immune system MESH: RNA medicine Humans MESH: HLA-A Antigens Corneal transplantation 030304 developmental biology HLA-G Antigens MESH: Pseudophakia MESH: Humans HLA-A Antigens Histocompatibility Antigens Class I MESH: Cornea MESH: Adult MESH: Immunohistochemistry medicine.disease eye diseases MESH: HLA-C Antigens HLA-B Antigens MESH: HLA-B Antigens RNA sense organs 030215 immunology |
| Zdroj: | Human Immunology Human Immunology, Elsevier, 2003, 64 (11), pp.1039-44 Human Immunology, 2003, 64 (11), pp.1039-44 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2003.08.346 |
| Popis: | Human leukocyte antigen (HLA)-G retains the capacity to modulate immune responses, favoring the establishment of tolerance in solid-tissue allotransplants. To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involved in corneal immunology. We therefore sought HLA-G expression in corneal tissues. Corneal transplantation consists in replacing the center of a diseased cornea with normal corneal tissue. Two corneal parts are not used in such surgery: diseased central corneal tissue and peripheral normal cornea. For this study, we used healthy corneas obtained from deceased donors and diseased corneas obtained from patients with pseudophakic bullous keratopathy or keratoconus who had undergone corneal transplantation. Immunohistochemical analysis carried out on the cryopreserved corneas showed a positive immunohistochemical staining with anti-HLA-G, anti-HLA-A, -B, and -C, and anti-HLA class I monoclonal antibodies. Staining was obtained for keratocytes, epithelial cells, and endothelial cells from both healthy and pathologic human corneas, revealing the presence of HLA class I proteins, including HLA-G. HLA-G transcripts were detected in normal cornea by reverse transcriptase-polymerase chain reaction with a classical pattern of alternative splicing. The detection of HLA-G protein in adult corneas leads to the conclusion that this protein may contribute to the maintenance of the privileged immune status of cornea. |
| Databáze: | OpenAIRE |
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