Analysis of degradation products of nerve agents via post-pentafluorobenzylation liquid chromatography-tandem mass spectrometry
| Autor: | Masanobu Uchiyama, Hajime Miyaguchi, Mai Otsuka, Yasuo Seto, Koichiro Tsuge |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Organophosphonates
02 engineering and technology Urinalysis Mass spectrometry Benzoates 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Organophosphorus Compounds Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry medicine Humans Chemical Warfare Agents Methylphosphonic acid Nerve agent Detection limit Chromatography 010401 analytical chemistry Organic Chemistry General Medicine Ethyl methylphosphonic acid 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Degradation (geology) Nerve Agents 0210 nano-technology Hydrophobic and Hydrophilic Interactions Blood Chemical Analysis Chromatography Liquid medicine.drug EMPA |
| Zdroj: | Journal of Chromatography A. 1577:31-37 |
| ISSN: | 0021-9673 |
| Popis: | In the work reported here, a screening procedure was developed for the detection and identification of RMPAs (nerve agent degradation products) after pentafluorobenzylation using liquid chromatography-tandem mass spectrometry (LC-MS/MS). With this method, all RMPAs, including highly hydrophilic types such as methylphosphonic acid (MPA) and ethyl methylphosphonic acid (EMPA), were sufficiently retained in commonly used reversed-phase columns (retention times: 15.7 and 11.0 min.), and the presence of RMPAs was determined more efficiently than with the conventional direct LC-MS/MS method. The detection limits of RMPAs using this approach (33 ng) were mostly superior to those observed with direct LC-MS/MS (74 ng) and gas chromatography-mass spectrometry (GC-MS) after pentafluorobenzylation (1.1 μg). The applicability of newly developed method toward real samples was evaluated via recovery tests involving urine/serum and wipe tests on various surfaces. |
| Databáze: | OpenAIRE |
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