Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells
| Autor: | Hisako Hikiji, Shirou Tabe, Yuji Seta, Takao Shimizu, Wataru Ariyoshi, Kazuhiro Tominaga, Tomomi Hashidate-Yoshida, Toshinori Okinaga, Tatsuji Nishihara, Hideo Shindou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cellular differentiation lcsh:Medicine Bone Morphogenetic Protein 2 Core Binding Factor Alpha 1 Subunit Phospholipase Glycerophospholipids Article Cell Line Mice 03 medical and health sciences Animals RNA Small Interfering lcsh:Science Messenger RNA Gene knockdown Multidisciplinary Chemistry lcsh:R Cell Differentiation 1-Acylglycerol-3-Phosphate O-Acyltransferase Chondrogenesis Cell biology 030104 developmental biology Cell culture Alkaline phosphatase lcsh:Q RNA Interference |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-16902-4 |
| Popis: | Glycerophospholipids have important structural and functional roles in cells and are the main components of cellular membranes. Glycerophospholipids are formed via the de novo pathway (Kennedy pathway) and are subsequently matured in the remodeling pathway (Lands’ cycle). Lands’ cycle consists of two steps: deacylation of phospholipids by phospholipases A2 and reacylation of lysophospholipids by lysophospholipid acyltransferases (LPLATs). LPLATs play key roles in the maturation and maintenance of the fatty acid composition of biomembranes, and cell differentiation. We examined whether LPLATs are involved in chondrogenic differentiation of ATDC5 cells, which can differentiate into chondrocytes. Lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards 18:1-, 18:2-, 20:4-, and 22:6-CoA increased in the late stage of chondrogenic differentiation, when mineralization occurred. LPCAT4 knockdown decreased mRNA and protein levels of chondrogenic markers as well as Alcian blue staining intensity and alkaline phosphatase activity in ATDC5 cells. These results suggest that LPCAT4 plays important roles during the transition of chondrocytes into hypertrophic chondrocytes and/or a mineralized phenotype. |
| Databáze: | OpenAIRE |
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