Maintenance of human adipose derived stem cell (hASC) differentiation capabilities using a 3D culture
| Autor: | Yuan-Kun Wu, Nai-Chen Cheng, Jiashing Yu, Chi-Hui Huang, Ching-Yu Lin |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cell Survival
Cell Cell Culture Techniques Adipose tissue Bioengineering Biology Applied Microbiology and Biotechnology Bioreactors Gene expression medicine Humans Confluency business.industry Stem Cells Microcarrier Cell Differentiation General Medicine Cell biology Biotechnology Staining medicine.anatomical_structure Adipose Tissue Adipogenesis Stem cell business |
| Zdroj: | Biotechnology Letters. 36:1529-1537 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-014-1500-y |
| Popis: | In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. Adipogenic or osteogenic differential medium was used to induce hASCs and differential potentials of these cells were compared between 2D and 3D environments via RT-PCR and staining quantifications. CEBP/α gene expression was significant higher in 3D condition at day 21 (P < 0.05). Staining quantification indicates that cells cultured in 3D condition have significant better differentiation potential from day 14 to 21 for both adipogenic and osteogenic lineages (P < 0.01). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink