ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range
| Autor: | Elena Rensen, Benoît Lelandais, Andrey Aristov, Christophe Zimmer |
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| Přispěvatelé: | Imagerie et Modélisation - Imaging and Modeling, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, This work was funded by Institut Pasteur, Région Ile de France (DIM Malinf), Fondation pour la Recherche Médicale (Equipe FRM, DEQ 20150331762), and Agence Nationale de la Recherche (grant ANR 14 CE10 0018 02). A.A. is supported by a Roux-Cantarini fellowship from Institut Pasteur., The authors thank M. Lelek, W. Ouyang and X. Hao for discussions and help on experimental and computational aspects, C. Leduc and C. Leterrier for help with microtubule immunolabeling, J. Bai for TOM22 antibodies, F. Di Nunzio for Nup133 antibodies, C. Sieben and S. Manley for sCMOS data., ANR-14-CE10-0018,HI-FISH,Etude systématique de l'expression des gènes à l'échelle des molécules uniques d'ARN(2014), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Single molecule localization Materials science Zernike polynomials Science General Physics and Astronomy Image processing General Biochemistry Genetics and Molecular Biology 03 medical and health sciences symbols.namesake Optics Microscopy lcsh:Science Range (particle radiation) Multidisciplinary Super-resolution microscopy business.industry Resolution (electron density) General Chemistry 030104 developmental biology symbols [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie lcsh:Q business 3d localization |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-8 (2018) Nature Communications Nature Communications, 2018, 9 (1), pp.2409. ⟨10.1038/s41467-018-04709-4⟩ Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.2409. ⟨10.1038/s41467-018-04709-4⟩ |
| ISSN: | 2041-1723 |
| Popis: | Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization Approach in 3D (ZOLA-3D), an easy-to-use computational and optical solution that achieves optimal resolution over a tunable axial range. We use ZOLA-3D to demonstrate 3D super-resolution imaging of mitochondria, nuclear pores and microtubules in entire nuclei or cells up to ~5 μm deep. |
| Databáze: | OpenAIRE |
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