Pre-therapy mRNA expression of TNF is associated with regimen-related gastrointestinal toxicity in patients with esophageal cancer: a pilot study
| Autor: | Joanne M. Bowen, Imogen A. White, Christos S. Karapetis, Dorothy M. K. Keefe, K Kristaly, H Tan, Tanya Irvine, David I. Watson, Lorelle Smith, Damian J. Hussey, Sarah K. Thompson, Anna Tsykin, Philip A. Game |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Oncology medicine.medical_specialty Esophageal Neoplasms Vomiting Nausea medicine.medical_treatment Antineoplastic Agents Pilot Projects Adenocarcinoma Real-Time Polymerase Chain Reaction Immune system Internal medicine Antineoplastic Combined Chemotherapy Protocols Biomarkers Tumor Mucositis medicine Humans RNA Messenger Chemotherapy Tumor Necrosis Factor-alpha business.industry Middle Aged Esophageal cancer medicine.disease Combined Modality Therapy Regimen Gamma Rays Toxicity Carcinoma Squamous Cell Female Fluorouracil Cisplatin medicine.symptom business |
| Zdroj: | Supportive Care in Cancer. 23:3165-3172 |
| ISSN: | 1433-7339 0941-4355 |
| Popis: | Esophageal cancer has a high mortality rate, and its multimodality treatment is often associated with significant rates of severe toxicity. Effort is needed to uncover ways to maximize effectiveness of therapy through identification of predictive markers of response and toxicity. As such, the aim of this study was to identify genes predictive of chemoradiotherapy-induced gastrointestinal toxicity using an immune pathway-targeted approach. Adults with esophageal cancer treated with chemotherapy consisting of 5-fluorouracil and cisplatin and 45–50 Gy radiation were recruited to the study. Pre-therapy-collected whole blood was analyzed for relative expression of immune genes using real-time polymerase chain reaction (RT-PCR). Gene expression was compared between patients who experienced severe regimen-related gastrointestinal toxicity vs. those experiencing mild to moderate toxicity. Blood from 31 patients were analyzed by RT-PCR. Out of 84 immune genes investigated, TNF was significantly elevated (2.05-fold, p = 0.025) in the toxic group (n = 12) compared to the non-toxic group (n = 19). Nausea and vomiting was the most commonly documented severe toxicity. No associations between toxicity and response, age, sex, histology, or treatment were evident. This study supports evidence of TNF as a predictive biomarker in regimen-related gastrointestinal toxicity. Confirming these findings in a larger cohort is warranted. |
| Databáze: | OpenAIRE |
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