Effects of 10-hydroxycamptothecin on intrinsic mitochondrial pathway in silkworm BmN-SWU1 cells
| Autor: | Chun Pan, Yan-Fen Hu, Yi-Ying Yang, Man Zhang, La Wang, Juan Song, Yong-Ping Wang, Cheng Lu, Hua-Shan Yi, Min-Hui Pan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
0301 basic medicine Health Toxicology and Mutagenesis Cell Apoptosis Mitochondrion 01 natural sciences Cell Line 03 medical and health sciences Botany medicine Animals biology Cytochrome c fungi Ovary General Medicine Bombyx Cell biology Mitochondria 010602 entomology 030104 developmental biology medicine.anatomical_structure Mitochondrial permeability transition pore Cell culture biology.protein DNA fragmentation Camptothecin Female Agronomy and Crop Science Intracellular |
| Zdroj: | Pesticide biochemistry and physiology. 127 |
| ISSN: | 1095-9939 |
| Popis: | 10-Hydroxycamptothecin (HCPT), a plant alkaloid isolated from Camptotheca acuminate, is known as a planted-derived insecticide, however, the specific mechanism in insect cells is still unclear. In this study, we treated the ovarian cell line of the silkworm, BmN-SWU1, with different HCPT doses for durations ranging from 0 to 72h. The apoptosis morphology was evident after 72h of incubation and included cell protuberance, concentrated cytoplasm and apoptotic bodies. We observed DNA fragmentation and cell apoptosis after HCPT treatment. The disruption of mitochondrial distribution, activation of the intracellular mitochondrial permeability transition pore, and release of cytochrome c during HCPT-induced apoptosis in dose and time-dependent manner indicate the involvement of mitochondria in BmN-SWU1 cells. Caspase-9 and -3 activities increased gradually with the duration of incubation time. In conclusion, HCPT has a significant effect to initiate the intrinsic mitochondrial pathway in silkworm cells, providing a theoretical basis for better application of plant-derived insecticide in pest control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect