A study on natural recovery of tassel fertilization and doubling method in maize haploids
| Autor: | P.K. Deng, X.Y. Yang, Lei Jiang, R.G. Zhao, Z. Xing, X.Y. Li, X.Q. Wang, G.X. Jing |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genotype Offspring Tassel Haploidy Biology Genes Plant Zea mays 01 natural sciences Chromosomes Plant 03 medical and health sciences Human fertilization Botany Genetics Plant breeding Inflorescence Molecular Biology Hybrid fungi General Medicine Plant Breeding Horticulture 030104 developmental biology Seeds Doubled haploidy Ploidy Colchicine 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Research. 16 |
| ISSN: | 1676-5680 |
| Popis: | Doubling method is the technical barriers in maize haploid breeding. It was very important to establish the independent intellectual property rights for doubling method. In this experiment, the maize haploid inducer, TG15, was used for producing maternal haploids. Also, haploids were obtained from two kinds of maternal genotypes involved in the experiment, including high-oil type and common type. Significant differences were observed among offspring of various genotypes in the recovery of haploid fertilization. In 21 hybrid offspring haploids, the average powder rate was 8.28%, and the seed setting rate was 4.98%. The experimental results showed that when the hybrids were treated with 0.08% colchicine, the average powder rate and seed setting rate of offspring haploids were 35.53 and 20.30%, respectively, which were significantly higher than the hybrids with natural recovery ability. This study primarily established the doubling method of haploids called "bud seedling method" in China which was very practicably in maize doubled haploid breeding. |
| Databáze: | OpenAIRE |
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