Differential expression of protein kinase C subtypes during ginsenoside Rh2-lnduced apoptosis in SK-N-BE(2) and C6Bu-1 cells
| Autor: | You Hiu Lee, Sung Ha Jin, Young Sook Kim, Jong Dae Park, Shin Il Kim |
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| Rok vydání: | 2000 |
| Předmět: |
Cell type
Ginsenosides Apoptosis Ginsenoside Rh2 Neuroblastoma Glioma Drug Discovery medicine Tumor Cells Cultured Animals Humans Protein kinase C Protein Kinase C Chemistry Organic Chemistry Saponins medicine.disease Molecular biology Cell biology Rats Isoenzymes Cell culture Molecular Medicine DNA fragmentation Drugs Chinese Herbal |
| Zdroj: | Archives of pharmacal research. 23(5) |
| ISSN: | 0253-6269 |
| Popis: | We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells. Apoptosis induced by G-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation and in situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes alpha, beta and gamma were progressively increased with prolonged treatment, whereas PKC delta increased transiently at 3 and 6 h and PKC epsilon was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype zeta markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-1 cells, no significant changes in PKC subtypes alpha, gamma, delta, epsilon and zeta were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types. |
| Databáze: | OpenAIRE |
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