Excision of uracil residues in DNA: mechanism of action ofEscherichia coliandMicrococcus luteusuracil-DNA glycosylases
| Autor: | Jacques Laval, Anne-Marie Delort, Robert Teoule, Jean-Pierre Leblanc, Anne-Marie Duplaa, D. Molko |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
DNA Repair
Micrococcus medicine.disease_cause DNA Glycosylases Substrate Specificity chemistry.chemical_compound Escherichia coli Genetics medicine heterocyclic compounds Uracil Uracil-DNA Glycosidase N-Glycosyl Hydrolases Base Sequence biology biology.organism_classification Oligodeoxyribonucleotides chemistry Biochemistry DNA glycosylase Uracil-DNA glycosylase Micrococcus luteus Cytosine DNA |
| Zdroj: | Nucleic Acids Research. 13:319-335 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/13.2.319 |
| Popis: | Various octadeoxynucleotides containing uracil at different positions were synthesized and submitted to the action of Escherichia coli and Micrococcus luteus uracil-DNA glycosylases. A uracil residue situated at the 5'-end was excised by the M.luteus enzyme but not by the E.coli one. Uracil residues located at the ultimate and penultimate positions at the 3'-end were not cleaved by either enzymes. At the other central positions, uracil was eliminated with different initial velocities. Single stranded phi X 174 DNA fragments were used to study the influence of the sequence. Cytosine bases were deaminated to give uracil by bisulfite treatment. It was shown that the initial excision velocity of two vicinal uracil residues was decreased. The same observation was made for two uracils separated by one base. A hypothetical scheme is suggested to explain the mechanism of action of uracil-DNA glycosylases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|