Molecular Mechanism of RNA Recognition by Zinc-Finger Antiviral Protein
| Autor: | Guangxia Gao, Songqing Liu, Yina Gao, Jingpeng Zhu, Xinlu Wang, Xiu Luo, Pu Gao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Models Molecular Guanine Antiviral protein chemical and pharmacologic phenomena ZINC FINGER ANTIVIRAL PROTEIN General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Protein Domains Animals Humans lcsh:QH301-705.5 Gene Zinc finger Base Sequence RNA RNA-Binding Proteins hemic and immune systems Cell biology 030104 developmental biology HEK293 Cells lcsh:Biology (General) chemistry Molecular mechanism RNA Viral Mutant Proteins 030217 neurology & neurosurgery Cytosine Protein Binding |
| Zdroj: | Cell Reports, Vol 30, Iss 1, Pp 46-52.e4 (2020) |
| ISSN: | 2211-1247 |
| Popis: | Summary: Zinc-finger antiviral protein (ZAP) is a host antiviral factor that specifically restricts a wide range of viruses. ZAP selectively binds to CG-dinucleotide-enriched RNA sequences and recruits multiple RNA degradation machines to degrade target viral RNA. However, the molecular mechanism and structural basis for ZAP recognition of specific RNA are not clear. Here, we report the crystal structure of the ZAP N-terminal domain bound to a CG-rich single-stranded RNA, providing the molecular basis for its specific recognition of a CG dinucleotide and additional guanine and cytosine. The four zinc fingers of ZAP adopt a unique architecture and form extensive interactions with RNA. Mutations of both protein and RNA at the RNA-ZAP interacting surface reduce the in vitro binding affinity and cellular antiviral activity. This work reveals the molecular mechanism of ZAP recognition of specific target RNA and also provides insights into the mechanism by which ZAP coordinates downstream RNA degradation processes. : ZAP is a host antiviral factor that specifically restricts a wide range of viruses. Luo et al. determine the structural and molecular basis for the specific recognition of a CG dinucleotide and additional guanine and cytosine by ZAP. Keywords: ▪▪▪ |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|