Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population ex vivo and in vitro
| Autor: | Elin Byman, Nina Schultz, Malin Wennström, Simon Moussaud, Kristoffer Brännström, Henrietta M. Nielsen, Anders Olofsson |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Aging Cell type amyloid‐beta 1‐40 Amyloid beta hippocampus Population Cell Culture Techniques Caspase 3 Biology Hippocampal formation Blood–brain barrier pericytes 03 medical and health sciences 0302 clinical medicine Journal Article medicine Humans Antigens education Aged Aged 80 and over education.field_of_study Amyloid beta-Peptides Neurosciences amyloid-beta 1-40 Cell Biology Original Articles Middle Aged Alzheimer's disease Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein Original Article Female Proteoglycans Pericyte 030217 neurology & neurosurgery Ex vivo Neurovetenskaper |
| Zdroj: | Aging Cell Aging Cell, 17:e12728. Wiley-Blackwell |
| ISSN: | 1474-9718 |
| Popis: | The population of brain pericytes, a cell type important for vessel stability and blood brain barrier function, has recently been shown altered in patients with Alzheimer's disease (AD). The underlying reason for this alteration is not fully understood, but progressive accumulation of the AD characteristic peptide amyloid-beta (Aβ) has been suggested as a potential culprit. In the current study, we show reduced number of hippocampal NG2+ pericytes and an association between NG2+ pericyte numbers and Aβ1-40 levels in AD patients. We further demonstrate, using in vitro studies, an aggregation-dependent impact of Aβ1-40 on human NG2+ pericytes. Fibril-EP Aβ1-40 exposure reduced pericyte viability and proliferation and increased caspase 3/7 activity. Monomer Aβ1-40 had quite the opposite effect: increased pericyte viability and proliferation and reduced caspase 3/7 activity. Oligomer-EP Aβ1-40 had no impact on either of the cellular events. Our findings add to the growing number of studies suggesting a significant impact on pericytes in the brains of AD patients and suggest different aggregation forms of Aβ1-40 as potential key regulators of the brain pericyte population size. Listed as co-author in the publication: The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam, The Netherlands |
| Databáze: | OpenAIRE |
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