Expression of CD95 on mature leukocytes of MRL/lpr mice after transplantation of genetically modified bone marrow stem cells
| Autor: | Hans-Uwe Simon, Shida Yousefi, Grace Gordon, Elena A. Federzoni, Inès Schmid, Stefan Müller |
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| Rok vydání: | 2008 |
| Předmět: |
Mice
Inbred MRL lpr T-Lymphocytes Genetic enhancement Immunology Gene Expression Bone Marrow Cells chemical and pharmacologic phenomena Biology Monocytes Mice Transduction Genetic hemic and lymphatic diseases Leukocytes medicine Animals Immunology and Allergy fas Receptor Autoimmune disease Lentivirus Hematopoietic Stem Cell Transplantation Bone Marrow Stem Cell hemic and immune systems Hematopoietic Stem Cells Fas receptor medicine.disease Transplantation Haematopoiesis Autoimmune lymphoproliferative syndrome Female Stem cell Granulocytes |
| Zdroj: | Immunology Letters. 117:45-49 |
| ISSN: | 0165-2478 |
| Popis: | Bone marrow transplantation (BMT) is commonly used for the treatment of severe haematological and immunological diseases. For instance, the autoimmune lymphoproliferative syndrome (ALPS) caused by a complete expression defect of CD95 (Fas, APO-1) can be cured by allogeneic BMT. However, since this therapy may not generate satisfactory results when only partially compatible donors are available, we were interested in the development of a potential alternative treatment by using lentiviral gene transfer of a normal copy of CD95 cDNA in hematopoietic stem cells. Here, we show that this approach applied to MRL/lpr mice results in the expression of functional CD95 receptors on the surface of lymphocytes, monocytes, and granulocytes. This suggests that correction of CD95 deficiency can be achieved by gene therapy. |
| Databáze: | OpenAIRE |
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