Resveratrol attenuates denervation-induced muscle atrophy due to the blockade of atrogin-1 and p62 accumulation
| Autor: | Masakazu Kinoshita, Kunihiro Sakuma, Miki Aizawa, Junji Ishikawa, Yuka Asami |
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| Rok vydání: | 2018 |
| Předmět: |
muscle atrophy
0301 basic medicine medicine.medical_specialty Normal diet Muscle Fibers Skeletal Muscle Proteins resveratrol Resveratrol 03 medical and health sciences chemistry.chemical_compound Gastrocnemius muscle Atrophy Internal medicine Stilbenes medicine Animals Humans Denervation atrogin-1 SKP Cullin F-Box Protein Ligases denervation business.industry p62 Skeletal muscle General Medicine medicine.disease Muscle Denervation Muscle atrophy Blockade Muscular Atrophy 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Dietary Supplements supplementation Mice Inbred mdx medicine.symptom business Transcription Factor TFIIH Signal Transduction Transcription Factors Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| DOI: | 10.7150/ijms.22723 |
| Popis: | Decrease in activity stress induces skeletal muscle atrophy. A previous study showed that treatment with resveratrol inhibits muscular atrophy in mdx mice, a model of DMD. However, almost all studies using resveratrol supplementation have only looked at adaptive changes in the muscle weight. The present study was designed to elucidate whether the resveratrol-inducing attenuation of skeletal muscle actually reflects the adaptation of muscle fibers themselves, based on the modulation of atrogin-1- or p62-dependent signaling. Mice were fed either a normal diet or 0.5% resveratrol diet. One week later, the right sciatic nerve was cut. The wet weight, mean fiber area, and amount of atrogin-1 and p62 proteins were examined in the gastrocnemius muscle at 14 days after denervation. The 0.5% resveratrol diet significantly prevented denervation-induced decreases in both the muscle weight and fiber atrophy. In addition, dietary resveratrol suppressed the denervation-induced atrogin-1 and p62 immunoreactivity. In contrast, 0.5% resveratrol supplementation did not significantly modulate the total protein amount of atrogin-1 or p62 in the denervated muscle of mice. Resveratrol supplementation significantly prevents muscle atrophy after denervation in mice, possibly due to the decrease in atrogin-1 and p62-dependent signaling. |
| Databáze: | OpenAIRE |
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