Long term exposure to environmental concentrations of diesel exhaust particles does not impact the phenotype of human bronchial epithelial cells

Autor: Nessrine Bellamri, Camille C Savary, Sophie Langouët, Laurent Vernhet, Valérie Lecureur, Claudie Morzadec
Přispěvatelé: Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Santé et de la Recherche Médicale, Université de Rennes 1, Jonchère, Laurent, Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Rok vydání: 2018
Předmět:
0301 basic medicine
Diesel exhaust
Bronchi
Toxicology
medicine.disease_cause
Cell morphology
complex mixtures
Cell Line
Histones
03 medical and health sciences
Bronchial epithelial cells
Cytochrome P-450 CYP1A1
medicine
Humans
Carcinogen
Vehicle Emissions
chemistry.chemical_classification
Reactive oxygen species
Toxicity
Cell growth
Epithelial Cells
General Medicine
respiratory system
Diesel exhaust particles
Molecular biology
Chronic exposure
respiratory tract diseases
3. Good health
[SDV.TOX] Life Sciences [q-bio]/Toxicology
Phenotype
030104 developmental biology
Gene Expression Regulation
chemistry
[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie
13. Climate action
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Cytochrome P-450 CYP1B1
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Reactive Oxygen Species
Genotoxicity
Oxidative stress
Zdroj: Toxicology in Vitro
Toxicology in Vitro, 2018, 52, pp.154-160. ⟨10.1016/j.tiv.2018.06.014⟩
Toxicology in Vitro, Elsevier, 2018, 52, pp.154-160. ⟨10.1016/j.tiv.2018.06.014⟩
ISSN: 0887-2333
DOI: 10.1016/j.tiv.2018.06.014
Popis: International audience; Chronic exposure to diesel engine exhausts is associated with an increased risk of pulmonary diseases including lung cancer. Diesel engine exhausts contain large amounts of diesel exhaust particles (DEP) on which are adsorbed several carcinogenic compounds such as polycyclic aromatic hydrocarbons. Acute toxicity of high concentrations of DEP has been largely demonstrated in various in vitro cellular models. In contrast, the cellular and molecular impacts of low environmental concentrations of DEP on the phenotype of chronically exposed lung epithelial cells remain to be investigated. In the present study, we show that long term exposure (6 months) to 2 μg/ml (0.4 μg/cm) DEP (standard reference material 1650b) increased cytochrome P4501A mRNA levels in the human bronchial epithelial BEAS-2B cell line. However, chronic exposure to DEP did not change cell morphology, trigger epithelial-mesenchymal transition or increase anchorage-independent cell growth. Moreover, DEP increase neither the levels of reactive oxygen species or those of γ-histone H2AX, nor the expression of interleukin-6 and interleukin-8. Our results thus demonstrate that the chronic exposure to low DEP concentrations could increase cytochrome P501A gene expression in BEAS-2B cells but did not induce molecular effects related to genotoxicity, oxidative stress or inflammation.
Databáze: OpenAIRE
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