Purification and primary structure of a necrosis-inducing peptide from the apoplastic fluids of tomato infected with Cladosporium fulvum (syn. Fulvia fulva)
| Autor: | I.M.J. Scholtens-Toma, P.J.G.M. de Wit |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
biology Protein primary structure food and beverages Peptide Plant Science Passalora fulva biology.organism_classification Amino acid Elicitor Laboratorium voor Phytopathologie Gene product chemistry Biochemistry Laboratory of Phytopathology Genetics Life Science Polyacrylamide gel electrophoresis Cladosporium |
| Zdroj: | Physiological and Molecular Plant Pathology, 33, 59-67 Physiological and Molecular Plant Pathology 33 (1988) |
| ISSN: | 0885-5765 |
| Popis: | Apoplastic fluids of compatible interactions between Cladosporium fulvum (syn. Fulvia fulva ) and tomato contain race-specific elicitors. The purification and final characterization of a peptide elicitor, the putative product of avirulence gene A9 of the fungus, which specifically induces necrosis in tomato cultivars containing the resistance gene Cf9 has been achieved. Cultivars with gene Cf9 were resistant to the fungal races used which contain avirulence gene A9 (races 4, 5 and 2.4) but susceptible to the race which lacked avirulence gene A9 (race 2.4.5.9). The peptide was purified to homogeneity by preparative polyacrylamide gel electrophoresis followed by reverse-phase HPLC. The complete sequence of the peptide was determined. The NH 2 -terminus was not blocked and the first amino acid was tyrosine. The total peptide contains 27 amino acids (3049 D), including six cysteine residues. |
| Databáze: | OpenAIRE |
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