Reply to van Swinderen and Hines: Drosophila model establishes the lipid membrane as a target of anesthetics
| Autor: | E. Nicholas Petersen, Richard A. Lerner, Scott B. Hansen, Mahmud Arif Pavel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Membranes Multidisciplinary biology Chemistry Stereochemistry Biological Sciences Anesthesia General biology.organism_classification Lipids 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Drosophila melanogaster Animals Drosophila Proteins lipids (amino acids peptides and proteins) Drosophila Letters Drosophila (subgenus) Lipid bilayer 030217 neurology & neurosurgery Anesthetics Neuroscience |
| Zdroj: | Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America |
| Popis: | We thank van Swinderen and Hines (1) for their response to our recent article, where we find that anesthetics disrupt lipid rafts and activate phospholipase D (PLD) through a membrane-mediated mechanism (2). Here we take the opportunity to address some minor misunderstandings and clarify our experimental procedures. Our chloroform induction in Drosophila is part of a larger study (3) which we reference in our study (reference 40 in ref. 2), referring the reader to a detailed description of the animal experiments, including the genotype of the wild-type flies (w1118), and full dose–response curves for three anesthetics (chloroform, isoflurane, … [↵][1]1To whom correspondence may be addressed. Email: shansen{at}scripps.edu or rlerner{at}scripps.edu. [1]: #xref-corresp-1-1 |
| Databáze: | OpenAIRE |
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