The Role of Transcription Factors in Gonad Development and Sex Differentiation of a Teleost Model Fish—Guppy (Poecilia reticulata)

Autor: Marek Skrobisz, Andrzej Przemysław Herman, Jakub Martynow, Kacper Kawalski, Hubert Szudrowicz, Maciej Kamaszewski, Adrian Szczepański, Maciej Wójcik, Patryk Bujarski
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Animals
Volume 10
Issue 12
Animals, Vol 10, Iss 2401, p 2401 (2020)
ISSN: 2076-2615
DOI: 10.3390/ani10122401
Popis: The guppy (Poecilia reticulata) is one of the world&rsquo
s most popular ornamental fish. Due to lecithotrophic viviparous, it is commonly used in toxicological studies and environmental monitoring. This study aimed to investigate the molecular mechanisms of gonad development and differentiation during guppy ontogenesis. The study mainly focused on the role and localization of potential specific sex markers and transcription factors: Sox9, Dmrt1, Er&beta
For histological analysis, guppies of both sexes were collected at 1, 60, and 360 dph (day post-hatching). The gonads morphology and immunohistochemistry detection of mentioned markers localization were performed. The expression of Sox9 protein was compared between sexes. Histological analysis revelated all types of male germinal cells in 60 dph guppy&rsquo
s testes. Maturated oocytes were visible in the ovaries of 360 dph fish. The Sox9 expression varied in spermatocytes and spermatids, from cellular to nuclear localization, and was higher in ovaries. Dmrt1 was detected in all testes groups and 360 dph ovaries. The Er&beta
was observed in both sexes at 60 and 360 dph. For the first time, the localization of transcription factors in guppy during ontogenesis was traced. The Sox9 designation as a factor regulating the development of germinal cells in adult guppies may facilitate the analysis of xenobiotics&rsquo
influence on fish&rsquo
s reproductive system.
Databáze: OpenAIRE
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