In vitro optimization of retinoic acid-induced neuritogenesis and TH endogenous expression in human SH-SY5Y neuroblastoma cells by the antioxidant Trolox
| Autor: | Alfeu Zanotto-Filho, Fábio Klamt, Ivi Juliana Bristot, Fares Zeidán-Chuliá, Daniel Pens Gelain, José Cláudio Fonseca Moreira, Matheus Augusto de Bittencourt Pasquali, André Simões Pires, Mario Luiz Conte da Frota Junior, Guilherme Antônio Behr, Fernanda Machado Lopes |
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| Rok vydání: | 2011 |
| Předmět: |
Antioxidant
Tyrosine 3-Monooxygenase Cell Survival medicine.medical_treatment Neurogenesis Clinical Biochemistry Retinoic acid Endogeny Tretinoin Biology Antioxidants chemistry.chemical_compound Neuroblastoma Phosphatidylinositol 3-Kinases Cell Line Tumor medicine Neurites Humans Microscopy Phase-Contrast Chromans Molecular Biology Protein kinase B Protein Kinase Inhibitors Phosphoinositide-3 Kinase Inhibitors chemistry.chemical_classification Reactive oxygen species Tyrosine hydroxylase Cell Biology General Medicine Cell biology Oxidative Stress chemistry Biochemistry Trolox Lipid Peroxidation Signal transduction Reactive Oxygen Species Proto-Oncogene Proteins c-akt |
| Zdroj: | Molecular and cellular biochemistry. 358(1-2) |
| ISSN: | 1573-4919 |
| Popis: | Though, it is quite well-known how retinoic acid (RA) is able to induce neuritogenesis in different in vitro models, the putative role exerted by reactive oxygen species (ROS) during this process still need to be further studied. For such purpose, we used a neuronal-like cell line (SH-SY5Y cells) in order to investigate whether the antioxidant Trolox (a hydrophilic analog of alpha-tocopherol) could have any effect on the number of RA-induced neurites, and how significant changes in cellular redox homeostasis may affect the cellular endogenous expression of tyrosine hydroxylase (TH). Our results show a significant enhancement of RA (10 μM)-induced neuritogenesis and TH endogenous expression, when cells were co-treated with Trolox (100 μM) for 7 days. Moreover, this effect was associated with an improvement in cellular viability. The mechanism seems to mainly involve PI3 K/Akt rather than MEK signaling pathway. Therefore, our data demonstrate that concomitant decreases in basal reactive oxygen species (ROS) production could exert a positive effect on the neuritogenic process of RA-treated SH-SY5Y cells. |
| Databáze: | OpenAIRE |
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