Effects of neuropeptides and sex steroids on the pituitary-gonadal axis of pre-pubertal F1 wreckfish (hāpuku) Polyprion oxygeneios in vivo: Evidence of inhibitory effects of androgens
| Autor: | Glen W. Irvine, Alvin N. Setiawan, Jane E. Symonds, Matthew J. Wylie, P. Mark Lokman, Abigail Elizur |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Polyprion oxygeneios Hypothalamo-Hypophyseal System medicine.drug_class Biology FSHB 03 medical and health sciences Endocrinology Kisspeptin Internal medicine medicine Animals Sexual Maturation Gonadal Steroid Hormones Fishes Pituitary gonadal axis biology.organism_classification 030104 developmental biology Glycoprotein Hormones alpha Subunit Androgens Hormone analog Animal Science and Zoology Female Gonadotropin Luteinizing hormone Hormone |
| Zdroj: | General and comparative endocrinology. 257 |
| ISSN: | 1095-6840 |
| Popis: | The ability to advance puberty in broodstock that have a long generation interval and mature at large size is a highly valuable tool in contemporary aquaculture enterprise. Juvenile male and female wreckfish 'hāpuku' (Polyprion oxygeneios), a candidate for commercialization in aquaculture, were subjected to treatment for 8weeks with two implants, one containing steroid (blank; estradiol-17β, E2; 11-ketotestosterone, KT; 17 α-methyltestosterone, MT), the other peptide (blank; gonadotropin-releasing hormone analog, GnRHa; kisspeptin, Kiss2-12). The expression of target genes (glycoprotein homone α-subunit, gpa; follicle stimulating-hormone β-subunit, fshb; luteinizing hormone β-subunit, lhb; GnRH receptor, gnrhr) in the pituitary was assayed by quantitative PCR. KT and MT decreased mRNA levels of all target genes in both male and female hāpuku, suggestive of a strong inhibitory tone by these steroid hormones. E2, GnRHa and Kiss2-12 were largely ineffective, regardless of whether they were administered alone or in combination with steroid implants. Clear differences in release and/or clearance rates between E2 and KT from implants were evident, in part explaining our observations. Advancement of puberty was not achieved, and we pose that different hormone doses and/or administration during more advanced stages of gonadogenesis need to be considered to move this field forward. |
| Databáze: | OpenAIRE |
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