Optical encoding of microbeads based on silica particle encapsulated quantum dots and its applications
| Autor: | Jianhao Wang, Hai-Qiao Wang, Jie Yang, Bo Liu, Yuan-Di Zhao, Yuan-Cheng Cao, Xin Jin, Liang Yang, Xiaoxia Zhu, Xiao-Feng Hua, Zhan Wang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Materials science Nanostructure Scanning electron microscope Mechanical Engineering technology industry and agriculture Bioengineering Nanotechnology General Chemistry Polymer equipment and supplies Colloid chemistry.chemical_compound chemistry Chemical engineering Mechanics of Materials Quantum dot Microscopy General Materials Science Microemulsion Polystyrene Electrical and Electronic Engineering |
| Zdroj: | Nanotechnology. 19(2) |
| ISSN: | 0957-4484 |
| Popis: | A novel method concerning the coding technology of polystyrene beads with Si encapsulated quantum dot (QD) particles (Si@QDs particles) is studied in this paper. In the reverse microemulsion system containing tetraethoxysilane (TEOS), water-soluble QDs (emission peak at 600 nm) were enveloped within the silica shell, forming Si@QDs particles. The Si@QDs particles were characterized by TEM, showing good uniform size, with an average diameter of about 167.0 nm. In comparison with the pure water-soluble QDs, the encapsulation of water-soluble QDs in the silica shell led to an enhancement in anti-photobleaching by providing inert barriers for the QDs. Images presented by SEM and confocal laser scanning microscopy demonstrated that the Si@QDs particles were equably coated on the surface of carboxyl functionalized polystyrene (PS) beads. Then, with the assistance of ethyl-3-(dimethyl aminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), human IgG could be successfully crosslinked to Si@QDs particle coated PS-COOH beads. Furthermore, the Si@QDs coated PS-COOH beads with human IgG were examined in immunoassay experiments, and the results indicated that these beads could be applied in the specific recognition of goat-anti-human IgG in solution. This investigation is expected to provide a new route to bead coding in the field of suspension microarrays, based on the use of QDs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|