Combination of uterine natural killer cell immunoglobulin receptor haplotype and trophoblastic HLA-C ligand influences the risk of pregnancy loss: a retrospective cohort analysis of direct embryo genotyping data from euploid transfers
| Autor: | Scott J. Morin, Nathan R. Treff, Xin Tao, Richard T. Scott, Jason M. Franasiak, Caroline R. Juneau, Marcy Maguire |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pregnancy Rate Biopsy Ligands HLA-C 0302 clinical medicine Receptors KIR Pregnancy Risk Factors Genotype 030219 obstetrics & reproductive medicine Homozygote Obstetrics and Gynecology Middle Aged Embryo transfer Trophoblasts Treatment Outcome embryonic structures Female Adult Heterozygote chemical and pharmacologic phenomena Fertilization in Vitro HLA-C Antigens Biology Risk Assessment Young Adult 03 medical and health sciences otorhinolaryngologic diseases medicine Humans Genetic Predisposition to Disease Allele Genotyping Retrospective Studies Ploidies Uterus Haplotype Embryo Transfer medicine.disease Abortion Spontaneous Pregnancy rate Blastocyst Fertility 030104 developmental biology Haplotypes Reproductive Medicine Infertility Immunology |
| Zdroj: | Fertility and Sterility. 107:677-683.e2 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2016.12.004 |
| Popis: | Objective To compare maternal uterine natural killer cell immunoglobulin receptor (KIR) genotype and haplotype frequencies between patients whose euploid single-embryo transfer resulted in pregnancy loss and those that resulted in delivery and to determine if the risk of pregnancy loss was affected by the HLA-C genotype content in the embryo. Design Retrospective cohort. Setting Academic research center. Patient(s) Autologous fresh IVF cycles resulting in positive serum β-hCG during 2009–2014. Intervention(s) None. Main outcome measure(s) 1) Relative risk of pregnancy loss according to maternal KIR genotypes and haplotypes. 2) Comparison of pregnancy loss rates within each KIR haplotype according to HLA-C ligand present in trophectoderm biopsy samples. Result(s) A total of 668 euploid single-embryo transfers with stored maternal DNA and available preamplification DNA from prior trophectoderm biopsy samples were studied. KIR2DS1, KIR3DS1, and KIR2DS5 were more common in patients who experienced pregnancy loss. Carriers of KIR A haplotype exhibited a decreased risk of pregnancy loss compared with KIR B haplotype carriers. However, among KIR A haplotype carriers, the risk of loss was significantly influenced by whether the transferred embryo carried a C1 allele versus no C1 alleles. Conclusion(s) KIR A haplotype carriers experienced fewer pregnancy losses than KIR B haplotype carriers after euploid single-embryo transfer. However, this risk was modified by HLA-C alleles present in the embryo. High-risk combinations (KIR A/homozygous C2 and KIR B/homozygous C1) resulted in a 51% increased risk of loss over all other combinations. |
| Databáze: | OpenAIRE |
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