Fluorimetric ex vivo quantification of protease debriding efficacy on natural substrate
| Autor: | Reddy Sreekanth Vootukuri, Michael P. Philpott, Giuseppe Trigiante |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteases
Bromelain (pharmacology) medicine.medical_treatment Dermatology Eschar 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine In vivo medicine Humans Skin Wound Healing Debridement biology Chemistry Treatment Outcome Biochemistry biology.protein Collagenase Surgery medicine.symptom Burns Elastin Ex vivo Biomarkers medicine.drug Peptide Hydrolases |
| Zdroj: | Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyREFERENCES. 28(6) |
| ISSN: | 1524-475X |
| Popis: | Debridement is the process of removal of necrotic and infected tissue to clean a wound or burn and expedite healing. Proteases such as papain, bromelain, and collagenase that promote debridement by degrading proteins in the dead tissue are in use today. However, the only method to measure debriding efficacy in vitro is the fluorescent monitoring of the digestion of an Artificial Wound Eschar (AWE) substrate. This AWE substrate contains a pellet of only three eschar matrix proteins collagen, elastin, and fibrin which do not account for the complexity and the composition of necrotic tissue. Here, we describe an ex vivo method using dry necrotic full thickness human skin and ortho-phthalaldehyde (OPA), a molecule commonly used for sensitive fluorimetric protein detection to monitor debridement activity. We advocate this simple yet sensitive approach to detect debridement efficacy that can readily be used commercially to benchmark products prior to in vivo testing. |
| Databáze: | OpenAIRE |
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