Humoral immunity in dually vaccinated SARS-CoV-2-naïve individuals and in booster-vaccinated COVID-19 convalescent subjects
| Autor: | Glück, Vivian, Tydykov, Leonid, Mader, Anna-Lena, Warda, Anne-Sophie, Bertok, Manuela, Weidlich, Tanja, Gottwald, Christine, Köstler, Josef, Salzberger, Bernd, Wagner, Ralf, Koller, Michael, Gessner, Andre, Schmidt, Barbara, Glück, Thomas, Peterhoff, David |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
ddc:610 SARS-CoV-2 610 Medizin COVID-19 General Medicine Antibodies Viral COVID-19 · SARS-CoV-2 · Antibody-mediated immunity · SARS-CoV-2-vaccination · Avidity · Surrogate neutralization Antibodies Neutralizing Immunity Humoral Infectious Diseases Immunoglobulin G Humans Angiotensin-Converting Enzyme 2 |
| Zdroj: | Infection, 50(6):1475-1481 |
| DOI: | 10.5283/epub.52151 |
| Popis: | Background The immune response to COVID-19-vaccination differs between naïve vaccinees and those who were previously infected with SARS-CoV-2. Longitudinal quantitative and qualitative serological differences in these two distinct immunological subgroups in response to vaccination are currently not well studied. Methods We investigate a cohort of SARS-CoV-2-naïve and COVID-19-convalescent individuals immediately after vaccination and 6 months later. We use different enzyme-linked immunosorbent assay (ELISA) variants and a surrogate virus neutralization test (sVNT) to measure IgG serum titers, IgA serum reactivity, IgG serum avidity and neutralization capacity by ACE2 receptor competition. Results Anti-receptor-binding domain (RBD) antibody titers decline over time in dually vaccinated COVID-19 naïves whereas titers in single dose vaccinated COVID-19 convalescents are higher and more durable. Similarly, antibody avidity is considerably higher among boosted COVID-19 convalescent subjects as compared to dually vaccinated COVID-19-naïve subjects. Furthermore, sera from boosted convalescents inhibited the binding of spike-protein to ACE2 more efficiently than sera from dually vaccinated COVID-19-naïve subjects. Conclusions Long-term humoral immunity differs substantially between dually vaccinated SARS-CoV-2-naïve and COVID-19-convalescent individuals. Booster vaccination after COVID-19 induces a more durable humoral immune response in terms of magnitude and quality as compared to two-dose vaccination in a SARS-CoV-2-naïve background. |
| Databáze: | OpenAIRE |
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