Arabidopsis AtERF71/HRE2 functions as transcriptional activator via cis-acting GCC box or DRE/CRT element and is involved in root development through regulation of root cell expansion
| Autor: | Eun Young Hwang, Vaishali N. Tarte, Se Bok Jang, Sun-Young Lee, Mi Suk Jeong, Hye-Yeon Seok, Yong-Hwan Moon |
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| Rok vydání: | 2014 |
| Předmět: |
biology
Arabidopsis Proteins Protoplasts Arabidopsis Electrophoretic Mobility Shift Assay Plant Science General Medicine urologic and male genital diseases biology.organism_classification Plant Roots Root cell Cell biology Cis acting Transactivation Seedlings Plant biochemistry Nucleotide Motifs Agronomy and Crop Science Transcriptional Activator Cell Proliferation Protein Binding Transcription Factors |
| Zdroj: | Plant cell reports. 34(2) |
| ISSN: | 1432-203X |
| Popis: | AtERF71/HRE2 binds to GCC box or DRE/CRT as transcription activator and plays an important role in root development via root cell expansion regulation. AtERF71/HRE2 transcription factor, a member of the AP2/ERF family, plays a key role in the stress response. GCC box and DRE/CRT, both essential cis-acting elements, have been shown to be recognized by AP2/ERF family transcription factors. However, it remains unclear whether or not AtERF71/HRE2 directly interacts with GCC box and/or DRE/CRT. Here, we showed that AtERF71/HRE2 binds to GCC box and DRE/CRT by electrophoretic mobility shift assay (EMSA). Binding of AtERF71/HRE2 to GCC box and DRE/CRT was also detected by fluorescence measurement and surface plasmon resonance spectroscopy (BIAcore) experiments. Folding properties of AtERF71/HRE2 proteins were characterized by CD spectroscopy, and AtERF71/HRE2 showed thermal stability as evidenced by two endothermic peaks (T d) at 53 and 65 °C. In addition, AtERF71/HRE2 showed transcriptional activation activity via GCC box and DRE/CRT in Arabidopsis protoplasts. Interestingly, AtERF71/HRE2 OXs showed increased primary root length due to elevated root cell expansion. Our data indicate that AtERF71/HRE2 binds to both GCC box and DRE/CRT, transactivates expression of genes downstream via GCC box or DRE/CRT, and plays an important role in root development through regulation of root cell expansion. |
| Databáze: | OpenAIRE |
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