Is expression of rat breast matrix components influenced by estrogen, progestins and tibolone?

Autor: T. R. Theodoro, César Eduardo Fernandes, A. M. A. A. Mader, E. R. A. Silveira, Maria Aparecida da Silva Pinhal, E P Cunha, V. F. Silva, Luciano de Melo Pompei, Marcelo Luis Steiner
Rok vydání: 2015
Předmět:
medicine.medical_specialty
Norpregnenes
medicine.drug_class
medicine.medical_treatment
Medroxyprogesterone Acetate
Tibolone
Dydrogesterone
Real-Time Polymerase Chain Reaction
Random Allocation
chemistry.chemical_compound
Estrogen Receptor Modulators
Proliferating Cell Nuclear Antigen
Internal medicine
medicine
Animals
Medroxyprogesterone acetate
Breast
Rats
Wistar
Cell Proliferation
Glucuronidase
Estradiol
biology
Progestogen
business.industry
Obstetrics and Gynecology
Estrogens
General Medicine
Immunohistochemistry
Norethisterone acetate
Extracellular Matrix
Rats
Proliferating cell nuclear antigen
Drug Combinations
Endocrinology
Matrix Metalloproteinase 9
chemistry
Estrogen
biology.protein
Estradiol benzoate
Matrix Metalloproteinase 2
Female
Norethindrone
Progestins
business
Biomarkers
Heparan Sulfate Proteoglycans
medicine.drug
Zdroj: Climacteric. 18:523-527
ISSN: 1473-0804
1369-7137
DOI: 10.3109/13697137.2015.1007122
Popis: To study the effects of estrogen therapy, alone or combined with progestogens, and of tibolone on the expression of heparanase (HSPE), extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), perlecan and proliferating cell nuclear antigen (PCNA) in normal breast tissue.Thirty 250-day-old Wistar rats were castrated and 3 weeks later received one of the following treatments by gavage for 5 weeks: (1) estradiol benzoate; (2) estradiol benzoate + medroxyprogesterone acetate; (3) estradiol benzoate + norethisterone acetate; (4) estradiol benzoate + dydrogesterone; (5) tibolone; (6) placebo. Following treatment, the expressions of mRNA for HSPE, MMP-2 and MMP-9 were analyzed by real-time PCR and the protein expressions of HSPE, MMP-2, MMP-9, perlecan and PCNA were quantified by immunohistochemistry.There was a statistically significant difference among the groups for the expression of HSPE mRNA due to high levels in the tibolone group. The groups differed in terms of PCNA, with lower levels found in the tibolone group followed by the estradiol benzoate + dydrogesterone group. A statistically significant positive correlation was observed for PCNA versus perlecan and MMP-9.There was no difference in the effects of combinations of estradiol and different progestogens on extracellular matrix components, and breast cell proliferation was associated with increases in perlecan and MMP-9.
Databáze: OpenAIRE
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