Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses
| Autor: | Ron D. Appel, Jean-Charles Sanchez, Céline Hernandez, Yohann Couté, Abelardo Margolles |
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| Přispěvatelé: | European Commission, Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Swiss National Science Foundation |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Sistemas biológicos
Proteomics Biotecnología Bifidobacterium longum Proteome Peptides/chemistry/metabolism Molecular Sequence Data Leucina Applied Microbiology and Biotechnology Bacterial Proteins/chemistry/metabolism Carbon Isotopes/chemistry/metabolism Isotope Labeling/methods fluids and secretions Bacterial Proteins Leucine Leucine/metabolism Methods Amino Acid Sequence ddc:576 Bifidobacterias Peptide sequence Bifidobacterium chemistry.chemical_classification Bacteriological Techniques Carbon Isotopes Ecology biology Bifidobacterium/growth & development/metabolism food and beverages biology.organism_classification Amino acid Culture Media Proteómica chemistry Biochemistry Isotope Labeling Actinomycetales Proteomics/methods Peptides Food Science Biotechnology |
| Zdroj: | Proteómica (03), 161 (2009) Helvia. Repositorio Institucional de la Universidad de Córdoba instname Applied and Environmental Microbiology, Vol. 73, No 17 (2007) pp. 5653-6 Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 0099-2240 |
| Popis: | Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence This work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06727-C02-01/ALI and fellowship PR2006-0421) and by the Swiss National Science Foundation (grant 3100-A0-104214). |
| Databáze: | OpenAIRE |
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