Comparative in vitro investigation of the cariogenic potential of bifidobacteria
| Autor: | Thais de Cássia Negrini, Karina Sampaio Caiaffa, Marcelle Danelon, Rodrigo Alex Arthur, Alberto Carlos Botazzo Delbem, Cristiane Duque, Remberto Marcelo Argandoña Valdez, V. R. Santos |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Faculty of Dentistry |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lactobacillus casei Bifidobacterium longum Virulence Factors 030106 microbiology Dental Caries In Vitro Techniques digestive system Streptococcus sobrinus Microbiology 03 medical and health sciences fluids and secretions Lactobacillus acidophilus Actinomyces Animals Biomass General Dentistry Tooth Demineralization Bifidobacterium biology Chemistry food and beverages Streptococcus Cell Biology General Medicine Hydrogen-Ion Concentration biology.organism_classification Bifidobacterium dentium Streptococcus mutans Bifidobacterium animalis Incisor stomatognathic diseases Lactobacillus Otorhinolaryngology Biofilms Dental caries Disease Progression bacteria Cattle |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1879-1506 |
| Popis: | Made available in DSpace on 2018-12-11T17:04:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective This study aimed to assess the in vitro cariogenic potential of some Bifidobacterium species in comparison with caries-associated bacteria. Design Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium animalis, Bifidobacterium dentium, Lactobacillus acidophilus, Lactobacillus casei, Actinomyces israelii, Streptococcus sobrinus and Streptococcus mutans were tested for acidogenicity and aciduricity by measuring the pH of the cultures after growth in glucose and bacterial growth after exposure to acid solutions. Biofilm biomass was determined for each species either alone or associated with S. mutans or S. mutans/S. sobrinus. Enamel hardness was analyzed before and after 7-days biofilm formation using bacterial combinations. Results B. animalis and B. longum were the most acidogenic and aciduric strains, comparable to caries-associated bacteria, such as S. mutans and L. casei. All species had a significantly increased biofilm when combined either with S. mutans or with S. mutans/S. sobrinus. The greatest enamel surface loss was produced when B. longum or B. animalis were inoculated with S. mutans, similar to L. casei and S. sobrinus. All strains induced similar enamel demineralization when combined with S. mutans/S. sobrinus, except by B. lactis. Conclusion The ability to produce acidic environments and to enhance biofilm formation leading to increased demineralization may mean that Bifidobacterium species, especially B. animalis and B. longum, are potentially cariogenic. UNESP - Univ. Estadual Paulista Araçatuba Dental School Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193, 16015050 UNESP - Univ. Estadual Paulista Araçatuba Dental School Department of Restorative Dentistry, Rua José Bonifácio, 1193, 16015050 UFRGS - Federal University of Rio Grande do Sul Faculty of Dentistry Department of Preventive Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003 UFRGS - Federal University of Rio Grande do Sul Faculty of Dentistry Department of Conservative Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003 UNESP - Univ. Estadual Paulista Araçatuba Dental School Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193, 16015050 UNESP - Univ. Estadual Paulista Araçatuba Dental School Department of Restorative Dentistry, Rua José Bonifácio, 1193, 16015050 FAPESP: #2012/19235-5 FAPESP: #2014/02072-1 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect