Characterization of MSB synapses in dissociated hippocampal culture with simultaneous pre- and postsynaptic live microscopy

Autor: Greg R. Phillips, Mónica Fernández-Monreal, Susan L. Wearne, Patrick R. Hof, James E. Reilly, Hugo H. Hanson
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Fluorescence-lifetime imaging microscopy
Dendritic spine
Time Factors
Synaptogenesis
Cell Culture Techniques
lcsh:Medicine
Hippocampal formation
Hippocampus
Biochemistry
law.invention
Rats
Sprague-Dawley
0302 clinical medicine
law
Postsynaptic potential
Pregnancy
Molecular Cell Biology
lcsh:Science
Neurons
0303 health sciences
Microscopy
Multidisciplinary
Neuronal Morphology
Neurochemistry
Anatomy
Animal Models
Female
Cellular Types
Research Article
animal structures
Cell Survival
Dendritic Spines
Neurogenesis
Presynaptic Terminals
Neuroimaging
Biology
03 medical and health sciences
Model Organisms
Developmental Neuroscience
Confocal microscopy
Live cell imaging
Animals
030304 developmental biology
Fluorescent Dyes
fungi
lcsh:R
Rats
Cellular Neuroscience
Synaptic plasticity
Biophysics
Rat
lcsh:Q
Neural Circuit Formation
030217 neurology & neurosurgery
Neuroscience
Synaptic Plasticity
Zdroj: PLoS ONE, Vol 6, Iss 10, p e26478 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the number of MSB synapses increases with synaptogenesis and plasticity but the formation, behavior, and fate of individual MSB synapses remains largely unknown. To address this, we developed a means of live imaging MSB synapses to observe them directly over time. With time lapse confocal microscopy of GFP-filled dendrites in contact with VAMP2-DsRed-labeled boutons, we recorded both MSBs and their contacting spines hourly over 15 or more hours. Our live microscopy showed that, compared to spines contacting single synaptic boutons (SSBs), MSB-contacting spines exhibit elevated dynamic behavior. These results are consistent with the idea that MSBs serve as intermediates in synaptic development and plasticity.
Databáze: OpenAIRE
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