Characterization of MSB synapses in dissociated hippocampal culture with simultaneous pre- and postsynaptic live microscopy
Autor: | Greg R. Phillips, Mónica Fernández-Monreal, Susan L. Wearne, Patrick R. Hof, James E. Reilly, Hugo H. Hanson |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Fluorescence-lifetime imaging microscopy
Dendritic spine Time Factors Synaptogenesis Cell Culture Techniques lcsh:Medicine Hippocampal formation Hippocampus Biochemistry law.invention Rats Sprague-Dawley 0302 clinical medicine law Postsynaptic potential Pregnancy Molecular Cell Biology lcsh:Science Neurons 0303 health sciences Microscopy Multidisciplinary Neuronal Morphology Neurochemistry Anatomy Animal Models Female Cellular Types Research Article animal structures Cell Survival Dendritic Spines Neurogenesis Presynaptic Terminals Neuroimaging Biology 03 medical and health sciences Model Organisms Developmental Neuroscience Confocal microscopy Live cell imaging Animals 030304 developmental biology Fluorescent Dyes fungi lcsh:R Rats Cellular Neuroscience Synaptic plasticity Biophysics Rat lcsh:Q Neural Circuit Formation 030217 neurology & neurosurgery Neuroscience Synaptic Plasticity |
Zdroj: | PLoS ONE, Vol 6, Iss 10, p e26478 (2011) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the number of MSB synapses increases with synaptogenesis and plasticity but the formation, behavior, and fate of individual MSB synapses remains largely unknown. To address this, we developed a means of live imaging MSB synapses to observe them directly over time. With time lapse confocal microscopy of GFP-filled dendrites in contact with VAMP2-DsRed-labeled boutons, we recorded both MSBs and their contacting spines hourly over 15 or more hours. Our live microscopy showed that, compared to spines contacting single synaptic boutons (SSBs), MSB-contacting spines exhibit elevated dynamic behavior. These results are consistent with the idea that MSBs serve as intermediates in synaptic development and plasticity. |
Databáze: | OpenAIRE |
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