Characterization of enteropathogenic Escherichia coli of clinical origin from the pediatric population in Pakistan
| Autor: | Oscar G. Gómez-Duarte, Syeda Sadia Bokhari, Zobia Noreen, Mahwish Younas, Brendan W. Wren, Habib Bokhari, Fariha Siddiqui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Diarrhea Nalidixic acid Tetracycline Virulence Factors 030106 microbiology Erythromycin Microbial Sensitivity Tests Biology Moths Infections Pediatrics Polymorphism Single Nucleotide beta-Lactamases Microbiology 03 medical and health sciences Enteropathogenic Escherichia coli Feces Antibiotic resistance Ampicillin medicine Animals Humans Pakistan Escherichia coli Infections Phylogeny Sulfamethoxazole Public Health Environmental and Occupational Health Infant Drug Resistance Microbial General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Trimethoprim Drug Resistance Multiple Anti-Bacterial Agents Disease Models Animal 030104 developmental biology Infectious Diseases Child Preschool bacteria Parasitology medicine.drug Plasmids |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 110(7) |
| ISSN: | 1878-3503 |
| Popis: | Enteropathogenic Escherichia coli (EPEC) is one of the leading causes of watery diarrhea among children. : In this study EPEC isolates from the pediatric population of Pakistan (2010-2012) were subjected to phylotyping, antibiotic susceptibility, extended-spectrum beta-lactamase (ESBL) profiling and evaluation of one representative strain from each panel of phylotypesin Galleria mellonella, infection model. : A total of 46/225 (20.4%) stool samples were positive for EPEC. Isolates mainly belong to D phylogroup (18, 39%) followed by nontypeable (10, 22%), B1 (9, 20%), B2 (8, 17%) and A (1, 2%). High resistance was observed for ampicillin (42, 91%), erythromycin (41, 89%), cefaclor (37, 80%), trimethoprim/sulfamethoxazole (36, 78%), tetracycline (36, 78%). Among nalidixic acid resistant isolates 13 (28%) showed presence of single nucleotide polymorphism (SNP) in parC (C330-T330) whereas 1 (2%) isolate showed gyrB (A660-T660) SNP. Furthermore, 27 (59%) isolates were ESBL producers. Representative isolates of phlyotypes A and B2 showed enhance killing of G. mellonella compared to ones belonging to phylotypes B1 and D. : Non-typeable EPEC strains were frequently observed. ESBL production in ESBL producers was found to be plasmid mediated. No significant association of antibiotic resistance profile with specific phylogroup of EPEC was found, however G. mellonella infection model differentiated representative phylotypes. |
| Databáze: | OpenAIRE |
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